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| Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production | |
| Gao, Baoyan1; Huang, Luodong1; Lei, Xueqing1; Meng, Ge1; Liu, Jianguo2,3; Zhang, Chengwu1 | |
| 2020-05-01 | |
| 发表期刊 | MARINE DRUGS
 |
| 卷号 | 18期号:5页码:17 |
| 通讯作者 | Zhang, Chengwu(tzhangcw@jnu.edu.cn) |
| 摘要 | Arachidonic acid (AA) is an omega-6 long-chain polyunsaturated fatty acid and is important for human health. The coccoid green microalga Lobosphaera bisecta has been reported to be able to accumulate high AA content under certain conditions. Nutrient management and light intensity had significant effects on the biomass and accumulation of lipids and AA in L. bisecta SAG2043. Both a high nitrogen concentration (18 mM) and high light intensity (bilateral light-300 mu mol m(-2) s(-1)) were beneficial to the growth of L. bisecta, and the replacement of culture medium further enhanced the biomass, which reached 8.9 g L-1. Low nitrogen concentration (3.6 mM) and high light significantly promoted the accumulation of lipids and AA. The highest lipid and AA content reached 54.0% and 10.8% of dry weight, respectively. Lipid compositions analysis showed that 88.2% of AA was distributed within the neutral lipids. We then reconstructed the lipid metabolic pathways of L. bisecta for the first time, and demonstrated that the upregulation of a key desaturase and elongase in the Delta 6 pathway was conducive to the accumulation of fatty acids toward AA synthesis. L. bisecta SAG2043 exhibits high biomass, lipid and AA production. It may be a potential candidate for AA production. |
| 关键词 | Lobosphaera bisecta light intensity medium replacement lipid arachidonic acid transcriptome analysis |
| DOI | 10.3390/md18050229 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | Sinopec joint program of China Petroleum and Chemical Corporation[ST18005-2] ; Guangzhou science and technology innovation program[201907010005] ; Fundamental Research Funds for the Central Universities[21619305] ; Blue Life of Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[MS2019N001] |
| WOS研究方向 | Pharmacology & Pharmacy |
| WOS类目 | Chemistry, Medicinal |
| WOS记录号 | WOS:000541031900007 |
| 出版者 | MDPI |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/167778 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Chengwu |
| 作者单位 | 1.Jinan Univ, Res Ctr Hydrobiol, Dept Ecol, Guangzhou 510632, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Baoyan,Huang, Luodong,Lei, Xueqing,et al. Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production[J]. MARINE DRUGS,2020,18(5):17. |
| APA | Gao, Baoyan,Huang, Luodong,Lei, Xueqing,Meng, Ge,Liu, Jianguo,&Zhang, Chengwu.(2020).Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production.MARINE DRUGS,18(5),17. |
| MLA | Gao, Baoyan,et al."Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production".MARINE DRUGS 18.5(2020):17. |
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| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Evaluation and Trans（2964KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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