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Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production
Gao, Baoyan1; Huang, Luodong1; Lei, Xueqing1; Meng, Ge1; Liu, Jianguo2,3; Zhang, Chengwu1
2020-05-01
Source PublicationMARINE DRUGS
Volume18Issue:5Pages:17
Corresponding AuthorZhang, Chengwu(tzhangcw@jnu.edu.cn)
AbstractArachidonic acid (AA) is an omega-6 long-chain polyunsaturated fatty acid and is important for human health. The coccoid green microalga Lobosphaera bisecta has been reported to be able to accumulate high AA content under certain conditions. Nutrient management and light intensity had significant effects on the biomass and accumulation of lipids and AA in L. bisecta SAG2043. Both a high nitrogen concentration (18 mM) and high light intensity (bilateral light-300 mu mol m(-2) s(-1)) were beneficial to the growth of L. bisecta, and the replacement of culture medium further enhanced the biomass, which reached 8.9 g L-1. Low nitrogen concentration (3.6 mM) and high light significantly promoted the accumulation of lipids and AA. The highest lipid and AA content reached 54.0% and 10.8% of dry weight, respectively. Lipid compositions analysis showed that 88.2% of AA was distributed within the neutral lipids. We then reconstructed the lipid metabolic pathways of L. bisecta for the first time, and demonstrated that the upregulation of a key desaturase and elongase in the Delta 6 pathway was conducive to the accumulation of fatty acids toward AA synthesis. L. bisecta SAG2043 exhibits high biomass, lipid and AA production. It may be a potential candidate for AA production.
KeywordLobosphaera bisecta light intensity medium replacement lipid arachidonic acid transcriptome analysis
DOI10.3390/md18050229
Indexed BySCI
Language英语
Funding ProjectSinopec joint program of China Petroleum and Chemical Corporation[ST18005-2] ; Guangzhou science and technology innovation program[201907010005] ; Fundamental Research Funds for the Central Universities[21619305] ; Blue Life of Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology[MS2019N001]
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000541031900007
PublisherMDPI
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167778
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Chengwu
Affiliation1.Jinan Univ, Res Ctr Hydrobiol, Dept Ecol, Guangzhou 510632, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China
Recommended Citation
GB/T 7714
Gao, Baoyan,Huang, Luodong,Lei, Xueqing,et al. Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production[J]. MARINE DRUGS,2020,18(5):17.
APA Gao, Baoyan,Huang, Luodong,Lei, Xueqing,Meng, Ge,Liu, Jianguo,&Zhang, Chengwu.(2020).Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production.MARINE DRUGS,18(5),17.
MLA Gao, Baoyan,et al."Evaluation and Transcriptome Analysis of the Novel Oleaginous Microalga Lobosphaera bisecta (Trebouxiophyceae, Chlorophyta) for Arachidonic Acid Production".MARINE DRUGS 18.5(2020):17.
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