Comprehensive Proteome Reveals the Key Lethal Toxins in the Venom of Jellyfish Nemopilema nomurai

doi:10.1021/acs.jproteome.0c00277

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	Comprehensive Proteome Reveals the Key Lethal Toxins in the Venom of Jellyfish Nemopilema nomurai
	Li, Rongfeng 1,2; Yu, Huahua1,2 ; Li, Tong 1; Li, Pengcheng1,2
	2020-06-05
发表期刊	JOURNAL OF PROTEOME RESEARCH
ISSN	1535-3893
卷号	19 期号:6 页码:2491-2500
通讯作者	Li, Rongfeng(rongfengli@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
摘要	Jellyfish stings are a major threat to human beings in coastal areas of the world. Each year, hundreds of thousands of victims are stung by venomous jellyfish. Nemopilema nomurai is a dangerous species with a large number of victims including many deaths. N. nomurai venom is a complex cocktail that is rich in proteins and peptides, and it is secreted by nematocysts for prey or defense. Previous studies have identified hundreds of toxins in the venom of N. nomurai; however, it is unclear which toxin(s) is responsible for lethality. Herein, we isolated the lethal fraction (NnLF) from N. nomurai venom with multiple chromatography. NnLF showed strong lethality to mice, and the toxicology results were consistent with the clinical symptoms of dead patients after N. nomurai sting, which indicated that NnLF contained the key lethal toxins in the venom. Subsequently, proteomic analysis was performed to identify the toxins in NnLF, and a total of 13 toxin homologues were identified, including phospholipase, potassium channel inhibitor, hemolysin, thrombin, etc. Moreover, in vitro toxicity assays further verified the phospholipase A(2) and hemolytic activity of NnLF. These results revealed that cell membrane-targeted toxins, including channel-forming toxins, potassium channel inhibitors, and especially phospholipases, played very important roles in the lethality of N. nomurai sting. Moreover, blood toxins such as thrombin-like toxin and hemolysins might be synergistically involved in lethality. These findings advance the understanding of lethality caused by N. nomurai sting and will be significant for the development of drugs to treat this jellyfish sting in the future.
关键词	jellyfish Nemopilema nomurai venom toxin NnLF
DOI	10.1021/acs.jproteome.0c00277
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[41876164] ; National Natural Science Foundation of China[41776163] ; Shandong Province Natural Science Foundation of China[ZR2019QD012] ; National Key RD Plan of China[2017YFE0111100-04]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000538772300027
出版者	AMER CHEMICAL SOC
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167682
专题	实验海洋生物学重点实验室
通讯作者	Li, Rongfeng; Li, Pengcheng
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
第一作者单位	中国科学院海洋大科学研究中心
通讯作者单位	中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Li, Rongfeng,Yu, Huahua,Li, Tong,et al. Comprehensive Proteome Reveals the Key Lethal Toxins in the Venom of Jellyfish Nemopilema nomurai[J]. JOURNAL OF PROTEOME RESEARCH,2020,19(6):2491-2500.
APA	Li, Rongfeng,Yu, Huahua,Li, Tong,&Li, Pengcheng.(2020).Comprehensive Proteome Reveals the Key Lethal Toxins in the Venom of Jellyfish Nemopilema nomurai.JOURNAL OF PROTEOME RESEARCH,19(6),2491-2500.
MLA	Li, Rongfeng,et al."Comprehensive Proteome Reveals the Key Lethal Toxins in the Venom of Jellyfish Nemopilema nomurai".JOURNAL OF PROTEOME RESEARCH 19.6(2020):2491-2500.

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Comprehensive Proteo（1746KB）	期刊论文	出版稿	限制开放	CC BY-NC-SA	浏览