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Comprehensive Proteome Reveals the Key Lethal Toxins in the Venom of Jellyfish Nemopilema nomurai
Li, Rongfeng1,2; Yu, Huahua1,2; Li, Tong1; Li, Pengcheng1,2
2020-06-05
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume19Issue:6Pages:2491-2500
Corresponding AuthorLi, Rongfeng(rongfengli@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
AbstractJellyfish stings are a major threat to human beings in coastal areas of the world. Each year, hundreds of thousands of victims are stung by venomous jellyfish. Nemopilema nomurai is a dangerous species with a large number of victims including many deaths. N. nomurai venom is a complex cocktail that is rich in proteins and peptides, and it is secreted by nematocysts for prey or defense. Previous studies have identified hundreds of toxins in the venom of N. nomurai; however, it is unclear which toxin(s) is responsible for lethality. Herein, we isolated the lethal fraction (NnLF) from N. nomurai venom with multiple chromatography. NnLF showed strong lethality to mice, and the toxicology results were consistent with the clinical symptoms of dead patients after N. nomurai sting, which indicated that NnLF contained the key lethal toxins in the venom. Subsequently, proteomic analysis was performed to identify the toxins in NnLF, and a total of 13 toxin homologues were identified, including phospholipase, potassium channel inhibitor, hemolysin, thrombin, etc. Moreover, in vitro toxicity assays further verified the phospholipase A(2) and hemolytic activity of NnLF. These results revealed that cell membrane-targeted toxins, including channel-forming toxins, potassium channel inhibitors, and especially phospholipases, played very important roles in the lethality of N. nomurai sting. Moreover, blood toxins such as thrombin-like toxin and hemolysins might be synergistically involved in lethality. These findings advance the understanding of lethality caused by N. nomurai sting and will be significant for the development of drugs to treat this jellyfish sting in the future.
Keywordjellyfish Nemopilema nomurai venom toxin NnLF
DOI10.1021/acs.jproteome.0c00277
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41876164] ; National Natural Science Foundation of China[41776163] ; Shandong Province Natural Science Foundation of China[ZR2019QD012] ; National Key RD Plan of China[2017YFE0111100-04]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000538772300027
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167682
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Rongfeng; Li, Pengcheng
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Rongfeng,Yu, Huahua,Li, Tong,et al. Comprehensive Proteome Reveals the Key Lethal Toxins in the Venom of Jellyfish Nemopilema nomurai[J]. JOURNAL OF PROTEOME RESEARCH,2020,19(6):2491-2500.
APA Li, Rongfeng,Yu, Huahua,Li, Tong,&Li, Pengcheng.(2020).Comprehensive Proteome Reveals the Key Lethal Toxins in the Venom of Jellyfish Nemopilema nomurai.JOURNAL OF PROTEOME RESEARCH,19(6),2491-2500.
MLA Li, Rongfeng,et al."Comprehensive Proteome Reveals the Key Lethal Toxins in the Venom of Jellyfish Nemopilema nomurai".JOURNAL OF PROTEOME RESEARCH 19.6(2020):2491-2500.
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