The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles

IOCAS-IR > 实验海洋生物学重点实验室

	The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles
	Wang, Y.1,3; Wang, B. J.1; Liu, M.1; Jiang, K. Y.1; Wang, M. Q.4,5; Wang, L.1,2,6
	2020
发表期刊	ISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN	1824-307X
卷号	17 页码:108-116
通讯作者	Wang, M. Q.(wangmengqiang@ouc.edu.cn) ; Wang, L.(leiwang@qdio.ac.cn)
摘要	As pattern recognition receptors (PRRs), C-type lectins (CTLs) have important roles in the recognition and clearance of pathogens by the innate immune system. In the present study, the first Cherax quadricarinatus CTL gene (designated CqCTL) was cloned and characterized. The complete cDNA sequence of CqCTL contained an open reading frame (ORF) of 543 bp, which encoded a protein of 180 amino acids. A carbohydrate recognition domain (CRD) containing four conserved cysteines (Cys(48), Cys(59), Cys(76), Cys(177)) and the EPD (Glu(80)-Pro(81)-Asn(82)) and QPD (Gln(146)-Pro(147)-Asn(148)) motifs were identified in the deduced amino acid sequence of CqCTL. The deduced tertiary structure of CqCTL revealed two a helices,five beta sheets and two disulfide bonds. CqCTL exhibited high similarity with previously identified CTLs from other species. The mRNA transcripts of CqCTL were ubiquitously detectable in all the tested tissues, with the highest expression level in hepatopancreas. These results provide useful information on the potential role of CqCTL in the innate immune system of C. quadricarinatus, and lay the foundation for further studies on the CTLs of crustacean.
关键词	Cherax quadricarinatus C-type lectin innate immunity
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2019YFD0900401] ; STS Program of Chinese Academy of Sciences, Fujian Provincial Department of Science and Technology[2019T3035]
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
WOS记录号	WOS:000536611100001
出版者	INVERTEBRATE SURVIVAL JOURNAL
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/167576
专题	实验海洋生物学重点实验室
通讯作者	Wang, M. Q.; Wang, L.
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Ocean Univ China, MOE Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China 5.Natl Lab Marine Sci & Technol, Ctr Marine Mol Biotechnol, Qingdao 266237, Peoples R China 6.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所; 中国科学院海洋大科学研究中心
推荐引用方式 GB/T 7714	Wang, Y.,Wang, B. J.,Liu, M.,et al. The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2020,17:108-116.
APA	Wang, Y.,Wang, B. J.,Liu, M.,Jiang, K. Y.,Wang, M. Q.,&Wang, L..(2020).The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles.ISJ-INVERTEBRATE SURVIVAL JOURNAL,17,108-116.
MLA	Wang, Y.,et al."The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles".ISJ-INVERTEBRATE SURVIVAL JOURNAL 17(2020):108-116.