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The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles
Wang, Y.1,3; Wang, B. J.1; Liu, M.1; Jiang, K. Y.1; Wang, M. Q.4,5; Wang, L.1,2,6
2020
Source PublicationISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN1824-307X
Volume17Pages:108-116
Corresponding AuthorWang, M. Q.(wangmengqiang@ouc.edu.cn) ; Wang, L.(leiwang@qdio.ac.cn)
AbstractAs pattern recognition receptors (PRRs), C-type lectins (CTLs) have important roles in the recognition and clearance of pathogens by the innate immune system. In the present study, the first Cherax quadricarinatus CTL gene (designated CqCTL) was cloned and characterized. The complete cDNA sequence of CqCTL contained an open reading frame (ORF) of 543 bp, which encoded a protein of 180 amino acids. A carbohydrate recognition domain (CRD) containing four conserved cysteines (Cys(48), Cys(59), Cys(76), Cys(177)) and the EPD (Glu(80)-Pro(81)-Asn(82)) and QPD (Gln(146)-Pro(147)-Asn(148)) motifs were identified in the deduced amino acid sequence of CqCTL. The deduced tertiary structure of CqCTL revealed two a helices,five beta sheets and two disulfide bonds. CqCTL exhibited high similarity with previously identified CTLs from other species. The mRNA transcripts of CqCTL were ubiquitously detectable in all the tested tissues, with the highest expression level in hepatopancreas. These results provide useful information on the potential role of CqCTL in the innate immune system of C. quadricarinatus, and lay the foundation for further studies on the CTLs of crustacean.
KeywordCherax quadricarinatus C-type lectin innate immunity
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2019YFD0900401] ; STS Program of Chinese Academy of Sciences, Fujian Provincial Department of Science and Technology[2019T3035]
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000536611100001
PublisherINVERTEBRATE SURVIVAL JOURNAL
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167576
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, M. Q.; Wang, L.
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Ocean Univ China, MOE Key Lab Marine Genet & Breeding, Qingdao 266003, Peoples R China
5.Natl Lab Marine Sci & Technol, Ctr Marine Mol Biotechnol, Qingdao 266237, Peoples R China
6.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao 266400, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences;  Center for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Y.,Wang, B. J.,Liu, M.,et al. The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2020,17:108-116.
APA Wang, Y.,Wang, B. J.,Liu, M.,Jiang, K. Y.,Wang, M. Q.,&Wang, L..(2020).The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles.ISJ-INVERTEBRATE SURVIVAL JOURNAL,17,108-116.
MLA Wang, Y.,et al."The first identification of a C-type lectin gene (CqCTL) in Cherax quadricarinatus: sequence features and expression profiles".ISJ-INVERTEBRATE SURVIVAL JOURNAL 17(2020):108-116.
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