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An insulin-like peptide serves as a regulator of glucose metabolism in the immune response of Chinese mitten crab Eriocheir sinensis
Wang, Lin1,3; Chen, Hao1; Wang, Lingling1,2,4; Song, Linsheng1,2,4
2020-07-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume108Pages:9
Corresponding AuthorSong, Linsheng(lshsong@dlou.edu.cn)
AbstractA robust immune response against invading pathogens greatly depends on the balance of metabolism, which could be vigorously modulated by insulin/IGF signaling (IIS) pathway in vertebrates. However, knowledge on the IIS pathway, especially the function of insulin-like peptides (ILPs) in invertebrates remained largely unknown. In the present study, a novel ILP was identified from Eriocheir sinensisis (designated EsILP). The coding sequence of EsILP was of 216 bp, which encoded a polypeptide of 71 amino acids containing an IlGF-like domain with four conserved cysteine residues. The mRNA transcripts of EsILP were found to be expressed dominantly in eyestalks and hepatopancreas, and EsILP protein was found to be distributed in the anterior median area of thoracic ganglion mass and the edges of hepatic tubules correspondingly. After Aeromonas hydrophila stimulation, EsILP transcripts were significantly increased at 3, 12 and 24 h post-stimulation in eyestalks and 6 and 48 h in hemocytes, respectively. In contrast, the expression level of EsILP decreased significantly in hepatopancreas from 6 h to 12 h after the stimulation. The glucose level in the hemolymph of crabs was significantly decreased from 6 to 12 h after the injection of recombinant EsILP. These results collectively demonstrated that the ancient ILP protein in E. sinensisis could negatively regulate glucose metabolism and participate in the immune response of the crabs against pathogen infection, which provided clues for the further investigation about the evolution and function of the IIS pathway in invertebrates.
KeywordAeromonas hydrophila infection Chinese mitten crab Glucose metabolism Insulin-like peptide Immune response
DOI10.1016/j.dci.2020.103686
Indexed BySCI
Language英语
Funding ProjectNational Key RD Program[2018YFD0900606] ; National Science Foundation of China[31530069] ; Fund for Outstanding Talents and Innovative Team of Agricultural Scientific Research ; Distinguished Professor of Liaoning ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000527338800002
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167236
Collection实验海洋生物学重点实验室
Corresponding AuthorSong, Linsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, Lin,Chen, Hao,Wang, Lingling,et al. An insulin-like peptide serves as a regulator of glucose metabolism in the immune response of Chinese mitten crab Eriocheir sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,108:9.
APA Wang, Lin,Chen, Hao,Wang, Lingling,&Song, Linsheng.(2020).An insulin-like peptide serves as a regulator of glucose metabolism in the immune response of Chinese mitten crab Eriocheir sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,108,9.
MLA Wang, Lin,et al."An insulin-like peptide serves as a regulator of glucose metabolism in the immune response of Chinese mitten crab Eriocheir sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 108(2020):9.
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