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Characteristics and sex dimorphism of 17 beta-hydroxysteroid dehydrogenase family genes in the olive flounder Paralichthys olivaceus
Zou, Congcong1,2,3; Wang, Lijuan1,2; Zou, Yuxia1,2; Wu, Zhihao1,2; Wang, Wenxiang1,2,3; Liang, Shaoshuai1,2; Wang, Ling1,2,3; You, Feng1,2
2020-05-01
Source PublicationJOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN0960-0760
Volume199Pages:17
Corresponding AuthorYou, Feng(youfeng@qdio.ac.cn)
AbstractSex steroid hormones play important roles in fish sex differentiation, gonadal development and secondary sexual characteristics. Olive flounder Paralichthys olivaceus is a valuable commercial marine fish species and has marked sexual dimorphism. However, the mechanisms of action of sex hormones in flounder sex are still unclear. In this study, a total of ten Hsd17b family genes, including Hsd17b3, -4, -7, -8, -9, -10, -12a, -12b, -14 and -15, were identified in the flounder, which encoded critical enzymes acting on sex steroid synthesis and metabolism. Hsd17b genes were distributed on eight chromosomes. Hsd17b12a and -12b were located on chromosomes 19 and 7, respectively. It was speculated that these two genes were just highly similar rather than different transcripts derived from the same gene. According to the results of domain and motif analyses, they all belonged to the SDR superfamily and contained conserved Hsd17b motifs TGxxxGxG, PGxxxT, NNAG and YxxxK. Analysis of amino acid sequences predicted that Hsd17b1, -4, -7, -12a and -14 were hydrophilic proteins. The stability of Hsd17b1, -3 and -12b proteins was predicted to be low. The various Hsd17b family genes differed in tissue expression pattern, and Hsd17b10, -12a and -12b were highly expressed in the flounder ovary. Moreover, throughout gonadal development, Hsd17b3 was highly expressed in the testis, and Hsd17b1, -12a and -12b were highly expressed in the ovary, suggesting that they might play an important role in testosterone synthesis in the testis or estrogen synthesis in the ovary. Activities of Hsd17b3 at stages I-V were all significantly higher in the testis than in the ovary (P < 0.05, P < 0.01). Transfection analysis in HEK293T cells showed that Hsd17b1 and -3 were located in both the cytoplasm and nucleus. Additionally, after challenging fish with tamoxifen, Hsd17b3 expression level in the testis decreased significantly (P < 0.01), and in the ovary no significant change was observed. Moreover, the expression of Hsd17b1 in the ovary was significantly upregulated after injection with flutamide (P < 0.05). These findings introduce the characteristics of the flounder Hsd17b in subfamily, which contribute to our understanding of the regulation of sex steroid hormone synthesis in fish gonadal development.
KeywordOlive flounder Paralichthys olivaceus Hsd17b Gene characteristics Expression Sex hormone receptor inhibitors Gonadal development
DOI10.1016/j.jsbmb.2020.105597
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0900202] ; National Natural Science Foundation of China[31772834] ; National Natural Science Foundation of China[31702337] ; National Natural Science Foundation of China[31872558] ; National Infrastructure of Fishery Germplasm Resources[2017DKA30470]
WOS Research AreaBiochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS SubjectBiochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS IDWOS:000526116100007
PublisherPERGAMON-ELSEVIER SCIENCE LTD
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/167134
Collection实验海洋生物学重点实验室
Corresponding AuthorYou, Feng
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Corresponding Author AffilicationCenter for Ocean Mega-Science, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zou, Congcong,Wang, Lijuan,Zou, Yuxia,et al. Characteristics and sex dimorphism of 17 beta-hydroxysteroid dehydrogenase family genes in the olive flounder Paralichthys olivaceus[J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY,2020,199:17.
APA Zou, Congcong.,Wang, Lijuan.,Zou, Yuxia.,Wu, Zhihao.,Wang, Wenxiang.,...&You, Feng.(2020).Characteristics and sex dimorphism of 17 beta-hydroxysteroid dehydrogenase family genes in the olive flounder Paralichthys olivaceus.JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY,199,17.
MLA Zou, Congcong,et al."Characteristics and sex dimorphism of 17 beta-hydroxysteroid dehydrogenase family genes in the olive flounder Paralichthys olivaceus".JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 199(2020):17.
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