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Full-length transcriptome sequencing and comparative transcriptomic analysis of different developmental stages of the sporophyll in Undaria pinnatifida (Laminariales: Alariaceae)
Shan, Tifeng1,2,3; Li, Qianxi1,2,3,4; Wang, Xuemei1,2,3,4; Pang, Shaojun1,2,3
2020-03-02
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Pages12
Corresponding AuthorShan, Tifeng(shantifeng@qdio.ac.cn) ; Pang, Shaojun(sjpang@qdio.ac.cn)
AbstractSporophyll development is an important biological process which is crucial to reproduction of the sporophyte of the brown alga Undaria pinnatifida. However, the molecular foundation underlying this process is largely unknown due to lack of genomic information in this important alga. Full-length transcriptome, which covered male and female gametophytes at vegetative and gametogenic phases, and the blade, stipe, immature and mature parts of the sporophyll of sporophytes, was obtained for the first time through single molecule real-time sequencing in this study. After removing potentially polluted sequences derived from microorganisms, we obtained a total of 61,181 transcripts with average and N50 length being 1639 and 1815 bp, respectively, and annotated 21,915 of them (35.8%). By using the full-length transcriptome as reference, we performed Illumina sequencing to analyze the difference of gene expression among different developmental stages of the sporophyll and validated the results by quantitative real-time PCR. A large number of transcripts related to flagellar components and fucoidan biosynthesis as well as a few transcripts coding for meiotic nuclear division protein were found to be significantly upregulated with development of the sporophyll, and thus the over-expression of them is likely essential for the sporophyll to perform its biological function. These findings contribute to our understanding of sporophyll development in U. pinnatifida at the transcriptional level.
KeywordPhaeophyceae SMRT sequencing Sporophyll development Flagellum Fucoidan Meiotic nuclear division
DOI10.1007/s10811-020-02080-w
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41676128] ; Biological Resources Program from Chinese Academy of Sciences[KFJ-BRP-017-27] ; China Agriculture Research System[CARS-50] ; Taishan Scholar Program of Shandong Province ; Foundation for Huiquan Scholar of Institute of Oceanology, Chinese Academy of Sciences
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000517681500001
PublisherSPRINGER
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165542
Collection实验海洋生物学重点实验室
Corresponding AuthorShan, Tifeng; Pang, Shaojun
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Shan, Tifeng,Li, Qianxi,Wang, Xuemei,et al. Full-length transcriptome sequencing and comparative transcriptomic analysis of different developmental stages of the sporophyll in Undaria pinnatifida (Laminariales: Alariaceae)[J]. JOURNAL OF APPLIED PHYCOLOGY,2020:12.
APA Shan, Tifeng,Li, Qianxi,Wang, Xuemei,&Pang, Shaojun.(2020).Full-length transcriptome sequencing and comparative transcriptomic analysis of different developmental stages of the sporophyll in Undaria pinnatifida (Laminariales: Alariaceae).JOURNAL OF APPLIED PHYCOLOGY,12.
MLA Shan, Tifeng,et al."Full-length transcriptome sequencing and comparative transcriptomic analysis of different developmental stages of the sporophyll in Undaria pinnatifida (Laminariales: Alariaceae)".JOURNAL OF APPLIED PHYCOLOGY (2020):12.
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