IOCAS-IR  > 海洋生态与环境科学重点实验室
Molecular method for rapid detection of the red tide dinoflagellate Karenia mikimotoi in the coastal region of Xiangshan Bay, China
Huang, Hai-Long1,2; Gao, Wei-Fang3; Zhu, Peng1,3; Zhou, Cheng-Xu1; Qiao, Long-Liang1; Dang, Chen-Yang1; Pang, Jian-Hu1; Yan, Xiao-Jun1
2020
Source PublicationJOURNAL OF MICROBIOLOGICAL METHODS
ISSN0167-7012
Volume168Pages:7
Corresponding AuthorZhu, Peng(zhupengnbu@nbu.edu.cn) ; Yan, Xiao-Jun(yanxiaojun@nbu.edu.cn)
AbstractThe species Karenia mikimotoi is a common nearshore red tide alga that can secrete hemolytic exotoxin and ichthyotoxin, which can induce the death of fish and shellfish, causing severe economic losses. In this study, loop-mediated isothermal amplification (LAMP) was employed in combination with the lateral flow dipstick (LFD) visual detection method to establish the LAMP-LFD rapid detection method for K. mikimotoi. The internal transcribed spacer ITS1-5.8S-ITS2 of K. mikimotoi was used as the target sequence and was amplified with specific primers designed in this study. The results indicated that the amplification optimal reaction conditions for LAMP in this paper were for 20 min at 65 degrees C. Moreover, LAMP had excellent specificity, showing negative results for other common red tide causing algal species. In field samples, we successfully reduced the total time, with only 23 min needed from LAMP amplification to LFD result display, which was shorter than that of conventional PCR. Consequently, LAMP-LFD should be useful for rapid field detection of low-density K. mikimotoi and for the early prevention of red tide induced by such algae.
KeywordKarenia mikimotoi ITS LAMP-LFD assay Rapid Harmful algae
DOI10.1016/j.mimet.2019.105801
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFD0900702] ; Zhejiang Provincial Public Welfare Technology Program of China[2017C33133] ; Ningbo Science and Technology Research Projects of China[2017C110003] ; China Spark Program of the National Ministry of Science and Technology[2015GA701001] ; Earmarked Fund for Modern Agro-industry Technology Research System, China[CARS-49] ; Scientific Research Foundation of Graduate School of Ningbo University ; Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Development Fund ; National 111 Project of China ; Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, China[LMEB201705] ; K.C. Wang Magna Fund in Ningbo University (SS)
WOS Research AreaBiochemistry & Molecular Biology ; Microbiology
WOS SubjectBiochemical Research Methods ; Microbiology
WOS IDWOS:000508748500014
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165311
Collection海洋生态与环境科学重点实验室
Corresponding AuthorZhu, Peng; Yan, Xiao-Jun
Affiliation1.Ningbo Univ, Key Lab Appl Marine Biotechnol, Ningbo 315211, Zhejiang, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China
3.Ningbo Inst Oceanog, Ningbo 315832, Zhejiang, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Huang, Hai-Long,Gao, Wei-Fang,Zhu, Peng,et al. Molecular method for rapid detection of the red tide dinoflagellate Karenia mikimotoi in the coastal region of Xiangshan Bay, China[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2020,168:7.
APA Huang, Hai-Long.,Gao, Wei-Fang.,Zhu, Peng.,Zhou, Cheng-Xu.,Qiao, Long-Liang.,...&Yan, Xiao-Jun.(2020).Molecular method for rapid detection of the red tide dinoflagellate Karenia mikimotoi in the coastal region of Xiangshan Bay, China.JOURNAL OF MICROBIOLOGICAL METHODS,168,7.
MLA Huang, Hai-Long,et al."Molecular method for rapid detection of the red tide dinoflagellate Karenia mikimotoi in the coastal region of Xiangshan Bay, China".JOURNAL OF MICROBIOLOGICAL METHODS 168(2020):7.
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