IOCAS-IR  > 海洋生态与环境科学重点实验室
Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process
Chai, Zhaoyang1; Hu, Zhangxi1,2,3; Liu, Yuyang1,4; Tang, Yingzhong1,2,3
2020
Source PublicationJOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
Volume38Issue:1Pages:114-123
Corresponding AuthorTang, Yingzhong(yingzhong.tang@qdio.ac.cn)
AbstractResting cysts play crucial roles in the ecology of dinoflagellates, especially in wintering or surviving unfavorable conditions, seeding harmful algal blooms (HABs), and facilitating the geographic expansion. Encystment of dinoflagellates is tightly coupled with sexual reproduction in most cases, which can occur either through homothallism (self-fertilization) or heterothallism (intercrossing of +/- strains). The types of sexual reproduction have important ecological implications. The toxic and HAB-forming dinoflagellate, Pheopolykrikoides hartmannii has been previously reported to be heterothallic. Here, we provide visual confirmation of homothally of P. hartmannii and the first detailed visual recording of cyst germination based on the observations of a clonal isolate from Jiaozhou Bay, China. To document the homothallism, we first observed cell pairs in sexual mating, planozygotes with two longitudinal flagella, and cysts with typical morphology as described previously from the clonal culture. We then germinated a single cyst, established a new clonal culture from one of the two daughter cells after the first cell division of the germling (i.e. from the diploid germling to two haploid cells), and produced cysts again from the newly established clonal culture. For the observation of the germination time-series, we took micrographs and videos to show all germination processes, particularly with an interesting observation of the short amoeboid stage of the germling releasing from the archeopyle (15 s), which was a landmark of the germination process and has not been reported elsewhere. This definitive evidence of homothallic sexuality and cyst production in P. hartmannii provides a new insight into the biology and ecology of the species, particularly a mechanism that may partly account for the population dynamics and ubiquitous distribution of the species.
Keywordgermination harmful algal blooms (HABs) heterothallism homothallism resting cyst Pheopolykrikos hartmannii
DOI10.1007/s00343-019-9077-x
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2017YFC1404300] ; National Natural Science Foundation of China[61533011] ; National Natural Science Foundation of China[41776125] ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; Science and Technology Basic Resources Investigation Program of China[2018FY100200] ; Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science[2016ASKJ02]
WOS Research AreaMarine & Freshwater Biology ; Oceanography
WOS SubjectLimnology ; Oceanography
WOS IDWOS:000511866000010
PublisherSCIENCE PRESS
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/165275
Collection海洋生态与环境科学重点实验室
Corresponding AuthorTang, Yingzhong
Affiliation1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chai, Zhaoyang,Hu, Zhangxi,Liu, Yuyang,et al. Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(1):114-123.
APA Chai, Zhaoyang,Hu, Zhangxi,Liu, Yuyang,&Tang, Yingzhong.(2020).Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(1),114-123.
MLA Chai, Zhaoyang,et al."Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.1(2020):114-123.
Files in This Item:
File Name/Size DocType Version Access License
Proof of homothally (1555KB)期刊论文出版稿开放获取CC BY-NC-SAView
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Chai, Zhaoyang]'s Articles
[Hu, Zhangxi]'s Articles
[Liu, Yuyang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Chai, Zhaoyang]'s Articles
[Hu, Zhangxi]'s Articles
[Liu, Yuyang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Chai, Zhaoyang]'s Articles
[Hu, Zhangxi]'s Articles
[Liu, Yuyang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Proof of homothally of Pheopolykrikos hartmannii and details of cyst germination process.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.