Institutional Repository of Key Laboratory of Marine Ecology & Environmental Sciences, CAS
| A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata | |
| Liu, Qin1,2,3,4; Guo, Yunhai1,2,3,4; Zhang, Yi1,2,3,4; Hu, Wei1,2,3,4,5; Li, Yuanyuan1,2,3,4; Zhu, Dan1,2,3,4; Zhou, Zhengbin1,2,3,4; Wu, Jiatong1,2,3,4; Chen, Nansheng6,7,8; Zhou, Xiao-Nong1,2,3,4 | |
| 2019-08-01 | |
| Source Publication | GIGASCIENCE
 |
| ISSN | 2047-217X |
| Volume | 8Issue:8Pages:8 |
| Corresponding Author | Chen, Nansheng(chennn@sfu.ca) ; Zhou, Xiao-Nong(xiaonongzhou1962@gmail.com) |
| Abstract | Background: Triatoma rubrofasciata is a widespread pathogen vector for Chagas disease, an illness that affects approximately 7 million people worldwide. Despite its importance to human health, its evolutionary origin has not been conclusively determined. A reference genome for T. rubrofasciata is not yet available. Finding: We have sequenced the genome of a female individual with T. rubrofasciata using a single molecular DNA sequencing technology (i.e., PacBio Sequel platform) and have successfully reconstructed a whole-genome (680-Mb) assembly that covers 90% of the nuclear genome (757 Mb). Through Hi-C analysis, we have reconstructed full-length chromosomes of this female individual that has 13 unique chromosomes (2n = 24 = 22 + X1 + X2) with a contig N50 of 2.72 Mb and a scaffold N50 of 50.7 Mb. This genome has achieved a high base-level accuracy of 99.99%. This platinum-grade genome assembly has 12,691 annotated protein-coding genes. More than 95.1% of BUSCO genes were single-copy completed, indicating a high level of completeness of the genome. Conclusion: The platinum-grade genome assembly and its annotation provide valuable information for future in-depth comparative genomics studies, including sexual determination analysis in T. rubrofasciata and the pathogenesis of Chagas disease. |
| Keyword | Triatoma rubrofasciata PacBio Sequel platform Hi-C chromosomal-level assembly comparative genomics RNA-Seq Iso-Seq |
| DOI | 10.1093/gigascience/giz089 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key Research and Development Program of China[2016YFC1202000] ; National Science and Technology Project[2018ZX10101002] ; CAS Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund |
| WOS Research Area | Life Sciences & Biomedicine - Other Topics ; Science & Technology - Other Topics |
| WOS Subject | Biology ; Multidisciplinary Sciences |
| WOS ID | WOS:000491875600004 |
| Publisher | OXFORD UNIV PRESS |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/164872 |
| Collection | 海洋生态与环境科学重点实验室 |
| Corresponding Author | Chen, Nansheng; Zhou, Xiao-Nong |
| Affiliation | 1.Chinese Ctr Dis Control & Prevent, Natl Inst Parasit Dis, Shanghai 200025, Peoples R China 2.Minist Hlth, Key Lab Parasite & Vector Biol, Shanghai 200025, Peoples R China 3.WHO Collaborating Ctr Trop Dis, Shanghai 200025, Peoples R China 4.Chinese Ctr Trop Dis Res, Shanghai 200025, Peoples R China 5.Fudan, Sch Life Sci, Dept Microbiol & Microbial Engn, Shanghai 200025, Peoples R China 6.Chinese Acad Sci, CAS Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China 7.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Shandong, Peoples R China 8.Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC, Canada |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Liu, Qin,Guo, Yunhai,Zhang, Yi,et al. A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata[J]. GIGASCIENCE,2019,8(8):8. |
| APA | Liu, Qin.,Guo, Yunhai.,Zhang, Yi.,Hu, Wei.,Li, Yuanyuan.,...&Zhou, Xiao-Nong.(2019).A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata.GIGASCIENCE,8(8),8. |
| MLA | Liu, Qin,et al."A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata".GIGASCIENCE 8.8(2019):8. |
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