Effects of Bacillus lincheniformis feeding frequency on the growth, digestion and immunity of Haliotis discus hannai

doi:10.1016/j.fsi.2019.11.038

IOCAS-IR > 实验海洋生物学重点实验室

	Effects of Bacillus lincheniformis feeding frequency on the growth, digestion and immunity of Haliotis discus hannai
	Gao Xiaolong1,2 ; Ke Caihuan 1; Wu Fucun 2; Li Xian 2; Liu Ying3
	2020
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	96 页码:1-12
通讯作者	Liu Ying(yingliu@dlou.edu.cn)
摘要	To study the effects of Bacillus lincheniformis feeding frequency on the survival and growth of Haliods discus hannai abalone, we measured the expression levels of nonspecific immune genes and monitored the anti-Vibrio parahaemolyticus immune reaction. H. discus hannai (shell length: 32.75 +/- 2.63 mm, body weight: 4.91 +/- 0.34 g) was selected to perform a 70 d laboratory culture experiment including a 14 d V. parahaemolyticus artificial infection experiment. The control group (C) was fed normal commercial feed every day. The M1 experimental group was given experimental feed and basal feed on alternating days until the end of the experiment. The M2 experimental group was given experimental feed for 4 d and basal feed for 3 d, and this cycle was repeated every 7 d until the end of the experiment. The M3 experimental group was given experimental feed for 2 d and basal feed for 5 d, and this cycle was repeated every 7 d until the end of the experiment. The M4 group was continuously given experimental feed for the duration of the experiment. The concentration of added B. lincheniformis in each experimental group was 10(5) cfu/g (according to the quantity of viable bacteria). The specific growth rate (as measured by body weight) and the feed conversion efficiency of the abalone in M1 and M2 were significantly higher than those in M4 and C (P < 0.05). The cellulose and lipase activities of abalone in M1, M2 or M4 were significantly higher than those in M3 or C (P < 0.05). The acid phosphatase, superoxide dismutase, total haemocyte counts, 02- levels generated by respiratory bursts, and the expression levels of Mn-SOD, TPx, GST(s), and GST(m), in abalone in the M2 group were significantly higher than those in any other feeding frequency group (P < 0.05). At the end of the V. parahaemolyticus infection, the cumulative mortality of the abalone in M2 was significantly lower than that in any other group (P < 0.05). Consequently, given the growth advantages and the enhancement of immune function, the feeding plan in which B. lincheniformis was applied for 4 d per week, and basal feed was then applied for 3 d, did not lead to a high level of immune reaction, immune fatigue or waste of resources, but increased the growth rate of individuals and their resistance to V. parahaemolyticus infection.
关键词	Bacillus lincheniformis Haliods discus hannai Feeding frequency Growth Immunity Disease resistance
DOI	10.1016/j.fsi.2019.11.038
收录类别	SCI
语种	英语
资助项目	earmarked funds for the Modern Agro-industry Technology Research System[CARS-49] ; China Postdoctoral Science Foundation[2019M650153] ; National Natural Science Foundation of China[31672673] ; Outstanding Postdoctoral Scholarship from the State Key Laboratory of Marine Environmental Science at Xiamen University ; earmarked funds for the Modern Agro-industry Technology Research System[CARS-49] ; China Postdoctoral Science Foundation[2019M650153] ; National Natural Science Foundation of China[31672673] ; Outstanding Postdoctoral Scholarship from the State Key Laboratory of Marine Environmental Science at Xiamen University
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000509617400001
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/164464
专题	实验海洋生物学重点实验室
通讯作者	Liu Ying
作者单位	1.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Dalian Ocean Univ, Dalian 116023, Peoples R China
第一作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Gao Xiaolong,Ke Caihuan,Wu Fucun,et al. Effects of Bacillus lincheniformis feeding frequency on the growth, digestion and immunity of Haliotis discus hannai[J]. FISH & SHELLFISH IMMUNOLOGY,2020,96:1-12.
APA	Gao Xiaolong,Ke Caihuan,Wu Fucun,Li Xian,&Liu Ying.(2020).Effects of Bacillus lincheniformis feeding frequency on the growth, digestion and immunity of Haliotis discus hannai.FISH & SHELLFISH IMMUNOLOGY,96,1-12.
MLA	Gao Xiaolong,et al."Effects of Bacillus lincheniformis feeding frequency on the growth, digestion and immunity of Haliotis discus hannai".FISH & SHELLFISH IMMUNOLOGY 96(2020):1-12.

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