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Effects of Bacillus lincheniformis feeding frequency on the growth, digestion and immunity of Haliotis discus hannai
Gao Xiaolong1,2; Ke Caihuan1; Wu Fucun2; Li Xian2; Liu Ying3
2020
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume96Pages:1-12
Corresponding AuthorLiu Ying(yingliu@dlou.edu.cn)
AbstractTo study the effects of Bacillus lincheniformis feeding frequency on the survival and growth of Haliods discus hannai abalone, we measured the expression levels of nonspecific immune genes and monitored the anti-Vibrio parahaemolyticus immune reaction. H. discus hannai (shell length: 32.75 +/- 2.63 mm, body weight: 4.91 +/- 0.34 g) was selected to perform a 70 d laboratory culture experiment including a 14 d V. parahaemolyticus artificial infection experiment. The control group (C) was fed normal commercial feed every day. The M1 experimental group was given experimental feed and basal feed on alternating days until the end of the experiment. The M2 experimental group was given experimental feed for 4 d and basal feed for 3 d, and this cycle was repeated every 7 d until the end of the experiment. The M3 experimental group was given experimental feed for 2 d and basal feed for 5 d, and this cycle was repeated every 7 d until the end of the experiment. The M4 group was continuously given experimental feed for the duration of the experiment. The concentration of added B. lincheniformis in each experimental group was 10(5) cfu/g (according to the quantity of viable bacteria). The specific growth rate (as measured by body weight) and the feed conversion efficiency of the abalone in M1 and M2 were significantly higher than those in M4 and C (P < 0.05). The cellulose and lipase activities of abalone in M1, M2 or M4 were significantly higher than those in M3 or C (P < 0.05). The acid phosphatase, superoxide dismutase, total haemocyte counts, 02- levels generated by respiratory bursts, and the expression levels of Mn-SOD, TPx, GST(s), and GST(m), in abalone in the M2 group were significantly higher than those in any other feeding frequency group (P < 0.05). At the end of the V. parahaemolyticus infection, the cumulative mortality of the abalone in M2 was significantly lower than that in any other group (P < 0.05). Consequently, given the growth advantages and the enhancement of immune function, the feeding plan in which B. lincheniformis was applied for 4 d per week, and basal feed was then applied for 3 d, did not lead to a high level of immune reaction, immune fatigue or waste of resources, but increased the growth rate of individuals and their resistance to V. parahaemolyticus infection.
KeywordBacillus lincheniformis Haliods discus hannai Feeding frequency Growth Immunity Disease resistance
DOI10.1016/j.fsi.2019.11.038
Indexed BySCI
Language英语
Funding Projectearmarked funds for the Modern Agro-industry Technology Research System[CARS-49] ; China Postdoctoral Science Foundation[2019M650153] ; National Natural Science Foundation of China[31672673] ; Outstanding Postdoctoral Scholarship from the State Key Laboratory of Marine Environmental Science at Xiamen University
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000509617400001
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164464
Collection实验海洋生物学重点实验室
Corresponding AuthorLiu Ying
Affiliation1.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
3.Dalian Ocean Univ, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Gao Xiaolong,Ke Caihuan,Wu Fucun,et al. Effects of Bacillus lincheniformis feeding frequency on the growth, digestion and immunity of Haliotis discus hannai[J]. FISH & SHELLFISH IMMUNOLOGY,2020,96:1-12.
APA Gao Xiaolong,Ke Caihuan,Wu Fucun,Li Xian,&Liu Ying.(2020).Effects of Bacillus lincheniformis feeding frequency on the growth, digestion and immunity of Haliotis discus hannai.FISH & SHELLFISH IMMUNOLOGY,96,1-12.
MLA Gao Xiaolong,et al."Effects of Bacillus lincheniformis feeding frequency on the growth, digestion and immunity of Haliotis discus hannai".FISH & SHELLFISH IMMUNOLOGY 96(2020):1-12.
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