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A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion
Li, Huili1,2,3; Sun, Boguang1,2; Ning, Xianhui1,2; Jiang, Shuai1,2; Sun, Li1,2
2019-11-01
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume20Issue:22Pages:20
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
AbstractEdwardsiella tarda is a Gram-negative bacterial pathogen with a broad host range, including fish, reptiles, and mammals. One prominent virulence feature of E. tarda is its ability to survive and replicate in host phagocytes, but the relevant molecular mechanism is largely unknown. In this study, we examined the transcriptome profiles of RAW264.7 cells, a murine macrophage cell line, infected with live E. tarda or stimulated with dead E. tarda for 4 h and 8 h. Eighteen libraries were constructed, and an average of 69 million clean reads per library were obtained, with similar to 81.63% of the reads being successfully mapped to the reference genome. In total, 208 and 232 differentially expressed genes (DEGs) were identified between live and dead E. tarda-treated cells at 4 h and 8 h post-infection, respectively. The DEGs were markedly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with immunity. Live E. tarda differed strikingly from dead E. tarda in the regulation of immune related genes. Compared with dead E. tarda-treated cells, live E. tarda-treated cells exhibited marked and significant suppression in the induction of a large amount of immune genes, including RIG-I-like receptors, cytokines, and interferon-related genes. Furthermore, some of the immune genes highly regulated by live E. tarda formed complicated interaction networks with each other. Together, the results of this study revealed a transcriptome profile specifically induced by the active virulence elements of live E. tarda during the infection process, thus adding new insights into the intracellular infection mechanism of E. tarda. This study also provided a valuable set of target genes for further study of the immune evasion strategy of E. tarda.
KeywordEdwardsiella tarda macrophage infection transcriptome immune evasion
DOI10.3390/ijms20225724
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0900500] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302-2] ; AoShan Talents Program ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Taishan Scholar Program of Shandong Province
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000502786800206
PublisherMDPI
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/164227
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1Wenhai Rd, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, 19 Yuquan Rd, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Huili,Sun, Boguang,Ning, Xianhui,et al. A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(22):20.
APA Li, Huili,Sun, Boguang,Ning, Xianhui,Jiang, Shuai,&Sun, Li.(2019).A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(22),20.
MLA Li, Huili,et al."A Comparative Analysis of Edwardsiella tarda-Induced Transcriptome Profiles in RAW264.7 Cells Reveals New Insights into the Strategy of Bacterial Immune Evasion".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.22(2019):20.
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