Institutional Repository of Key Laboratory of Marine Environmental Corrosion and Bio-fouling, IOCAS
Determination of sulfate-reducing bacteria with chemical conversion from ZnO nanorods arrays to ZnS arrays | |
Qi, Peng1,2; Zhang, Dun1; Wan, Yi1,2; Zhang, D | |
2013-05-01 | |
发表期刊 | SENSORS AND ACTUATORS B-CHEMICAL |
ISSN | 0925-4005 |
卷号 | 181页码:274-279 |
文章类型 | Article |
摘要 | This work presents a novel specific detection method for sulfate-reducing bacteria (SRB) based on sulfuration conversion from ZnO arrays to ZnS arrays. The sulfuration process was conducted in two steps. First, ZnO@ZnS intermediate nanocomposite arrays were formed by the sulfuration reaction between ZnO and sulfide, which is the characteristic enzymatic metabolism product of SRB. Next, unconverted ZnO cores were washed away in ammonia buffer solutions, leaving ZnS arrays on the surface of ITO substrates. The array conversion process can be used for SRB detection because the final state of ZnS arrays was highly related with the amount of accumulated sulfide, which was decided by the initial concentration of SRB. Electrochemical impedance spectroscopy has been used to optimize experimental conditions and detect SRB in aqueous solutions. This SRB detection method based on arrays conversion from ZnO to ZnS can avoid the use of biological recognition elements, which are expensive and easy to lose specific recognizing abilities. In addition, compared with the widely used MPN method which would take up to 15 days to accomplish the detection process, the proposed method can shorten the detection time greatly. (C) 2013 Elsevier B.V. All rights reserved.; This work presents a novel specific detection method for sulfate-reducing bacteria (SRB) based on sulfuration conversion from ZnO arrays to ZnS arrays. The sulfuration process was conducted in two steps. First, ZnO@ZnS intermediate nanocomposite arrays were formed by the sulfuration reaction between ZnO and sulfide, which is the characteristic enzymatic metabolism product of SRB. Next, unconverted ZnO cores were washed away in ammonia buffer solutions, leaving ZnS arrays on the surface of ITO substrates. The array conversion process can be used for SRB detection because the final state of ZnS arrays was highly related with the amount of accumulated sulfide, which was decided by the initial concentration of SRB. Electrochemical impedance spectroscopy has been used to optimize experimental conditions and detect SRB in aqueous solutions. This SRB detection method based on arrays conversion from ZnO to ZnS can avoid the use of biological recognition elements, which are expensive and easy to lose specific recognizing abilities. In addition, compared with the widely used MPN method which would take up to 15 days to accomplish the detection process, the proposed method can shorten the detection time greatly. (C) 2013 Elsevier B.V. All rights reserved. |
关键词 | Detection Zno Arrays Zns Arrays Sulfate-reducing Bacteria |
学科领域 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
DOI | 10.1016/j.snb.2013.01.076 |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
WOS类目 | Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation |
WOS记录号 | WOS:000317941100039 |
WOS关键词 | SENSITIZED SOLAR-CELLS ; NANOWIRE ARRAYS ; NANOSTRUCTURES ; GROWTH |
WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qdio.ac.cn/handle/337002/16360 |
专题 | 海洋环境腐蚀与生物污损重点实验室 |
通讯作者 | Zhang, D |
作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Natl Engn Res Ctr Marine Corros Protect, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China |
第一作者单位 | 中国科学院海洋研究所 |
推荐引用方式 GB/T 7714 | Qi, Peng,Zhang, Dun,Wan, Yi,et al. Determination of sulfate-reducing bacteria with chemical conversion from ZnO nanorods arrays to ZnS arrays[J]. SENSORS AND ACTUATORS B-CHEMICAL,2013,181:274-279. |
APA | Qi, Peng,Zhang, Dun,Wan, Yi,&Zhang, D.(2013).Determination of sulfate-reducing bacteria with chemical conversion from ZnO nanorods arrays to ZnS arrays.SENSORS AND ACTUATORS B-CHEMICAL,181,274-279. |
MLA | Qi, Peng,et al."Determination of sulfate-reducing bacteria with chemical conversion from ZnO nanorods arrays to ZnS arrays".SENSORS AND ACTUATORS B-CHEMICAL 181(2013):274-279. |
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