Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis

doi:10.1016/j.gene.2019.05.021

IOCAS-IR > 实验海洋生物学重点实验室

	Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis
	Du, Jing 1,4; Liu, Yuan 1; Song, Chengwen1,4 ; Cui, Zhaoxia 2,3
	2019-08-20
发表期刊	GENE
ISSN	0378-1119
卷号	710 页码:1-8
通讯作者	Cui, Zhaoxia(cuizhaoxia@nbu.edu.cn)
摘要	Investigation of sex determination system in Eriocheir sinensis is important because of sex-dimorphism in its growth traits. However, little information about the sex-related genes in embryonic development stages were exposed. To obtain more information of sex determination in Chinese mitten crab, we performed the transcriptome analysis in embryonic development stage (fertilized egg stage, cleavage stage, blastula stage, gastrula stage and heartbeat stage) of Chinese mitten crab using nextgeneration sequencing technology. Thirty-one of 32,088 annotated unigenes were identified as sex-related genes including 16 genes involved in primary sex determination in model organisms and 8 genes of SOX family and 7 genes of DMRT gene family. Heatmap based on the RPKM value in five embryonic development stages indicated that these genes were clustered into two branches. Analysis of the differentially expressed 12 genes, including 3 genes of SOX family, 3 genes of DMRT gene family and 6 genes involved in primary sex determination in model organisms, showed significantly difference between the first three periods (fertilized egg stage-cleavage stage-blastula stage) and the last two periods (gastrula stage-heartbeat stage) and all 12 genes were up-regulated after blastula stage. In conclusion, we inferred that sex determination might be initiated after blastula stage in E. sinensis. Transcriptome analysis from embryonic development stage could provide a background information for further investigation in sex determination of Eriocheir sinensis.
关键词	Transcriptome Sex determination Embryonic development Eriocheir sinensis
DOI	10.1016/j.gene.2019.05.021
收录类别	SCI
语种	英语
资助项目	National Key Research and Development Program of China[2018YFD0900303] ; Ten Thousand Talents Program ; National Key Research and Development Program of China[2018YFD0900303] ; Ten Thousand Talents Program
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000483423500001
出版者	ELSEVIER
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/162865
专题	实验海洋生物学重点实验室
通讯作者	Cui, Zhaoxia
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Ningbo Univ, Ningbo 315020, Zhejiang, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Du, Jing,Liu, Yuan,Song, Chengwen,et al. Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis[J]. GENE,2019,710:1-8.
APA	Du, Jing,Liu, Yuan,Song, Chengwen,&Cui, Zhaoxia.(2019).Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis.GENE,710,1-8.
MLA	Du, Jing,et al."Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis".GENE 710(2019):1-8.

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