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Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis
Du, Jing1,4; Liu, Yuan1; Song, Chengwen1,4; Cui, Zhaoxia2,3
2019-08-20
Source PublicationGENE
ISSN0378-1119
Volume710Pages:1-8
Corresponding AuthorCui, Zhaoxia(cuizhaoxia@nbu.edu.cn)
AbstractInvestigation of sex determination system in Eriocheir sinensis is important because of sex-dimorphism in its growth traits. However, little information about the sex-related genes in embryonic development stages were exposed. To obtain more information of sex determination in Chinese mitten crab, we performed the transcriptome analysis in embryonic development stage (fertilized egg stage, cleavage stage, blastula stage, gastrula stage and heartbeat stage) of Chinese mitten crab using nextgeneration sequencing technology. Thirty-one of 32,088 annotated unigenes were identified as sex-related genes including 16 genes involved in primary sex determination in model organisms and 8 genes of SOX family and 7 genes of DMRT gene family. Heatmap based on the RPKM value in five embryonic development stages indicated that these genes were clustered into two branches. Analysis of the differentially expressed 12 genes, including 3 genes of SOX family, 3 genes of DMRT gene family and 6 genes involved in primary sex determination in model organisms, showed significantly difference between the first three periods (fertilized egg stage-cleavage stage-blastula stage) and the last two periods (gastrula stage-heartbeat stage) and all 12 genes were up-regulated after blastula stage. In conclusion, we inferred that sex determination might be initiated after blastula stage in E. sinensis. Transcriptome analysis from embryonic development stage could provide a background information for further investigation in sex determination of Eriocheir sinensis.
KeywordTranscriptome Sex determination Embryonic development Eriocheir sinensis
DOI10.1016/j.gene.2019.05.021
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2018YFD0900303] ; Ten Thousand Talents Program
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000483423500001
PublisherELSEVIER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162865
Collection实验海洋生物学重点实验室
Corresponding AuthorCui, Zhaoxia
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Ningbo Univ, Ningbo 315020, Zhejiang, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Du, Jing,Liu, Yuan,Song, Chengwen,et al. Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis[J]. GENE,2019,710:1-8.
APA Du, Jing,Liu, Yuan,Song, Chengwen,&Cui, Zhaoxia.(2019).Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis.GENE,710,1-8.
MLA Du, Jing,et al."Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis".GENE 710(2019):1-8.
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