Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis | |
Du, Jing1,4; Liu, Yuan1; Song, Chengwen1,4 ; Cui, Zhaoxia2,3
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| 2019-08-20 | |
| Source Publication | GENE
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| ISSN | 0378-1119 |
| Volume | 710Pages:1-8 |
| Corresponding Author | Cui, Zhaoxia(cuizhaoxia@nbu.edu.cn) |
| Abstract | Investigation of sex determination system in Eriocheir sinensis is important because of sex-dimorphism in its growth traits. However, little information about the sex-related genes in embryonic development stages were exposed. To obtain more information of sex determination in Chinese mitten crab, we performed the transcriptome analysis in embryonic development stage (fertilized egg stage, cleavage stage, blastula stage, gastrula stage and heartbeat stage) of Chinese mitten crab using nextgeneration sequencing technology. Thirty-one of 32,088 annotated unigenes were identified as sex-related genes including 16 genes involved in primary sex determination in model organisms and 8 genes of SOX family and 7 genes of DMRT gene family. Heatmap based on the RPKM value in five embryonic development stages indicated that these genes were clustered into two branches. Analysis of the differentially expressed 12 genes, including 3 genes of SOX family, 3 genes of DMRT gene family and 6 genes involved in primary sex determination in model organisms, showed significantly difference between the first three periods (fertilized egg stage-cleavage stage-blastula stage) and the last two periods (gastrula stage-heartbeat stage) and all 12 genes were up-regulated after blastula stage. In conclusion, we inferred that sex determination might be initiated after blastula stage in E. sinensis. Transcriptome analysis from embryonic development stage could provide a background information for further investigation in sex determination of Eriocheir sinensis. |
| Keyword | Transcriptome Sex determination Embryonic development Eriocheir sinensis |
| DOI | 10.1016/j.gene.2019.05.021 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Key Research and Development Program of China[2018YFD0900303] ; Ten Thousand Talents Program ; National Key Research and Development Program of China[2018YFD0900303] ; Ten Thousand Talents Program |
| WOS Research Area | Genetics & Heredity |
| WOS Subject | Genetics & Heredity |
| WOS ID | WOS:000483423500001 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/162865 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Cui, Zhaoxia |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Ningbo Univ, Ningbo 315020, Zhejiang, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Du, Jing,Liu, Yuan,Song, Chengwen,et al. Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis[J]. GENE,2019,710:1-8. |
| APA | Du, Jing,Liu, Yuan,Song, Chengwen,&Cui, Zhaoxia.(2019).Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis.GENE,710,1-8. |
| MLA | Du, Jing,et al."Discovery of sex-related genes from embryonic development stage based on transcriptome analysis in Eriocheir sinensis".GENE 710(2019):1-8. |
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| File Name/Size | DocType | Version | Access | License | ||
| 1-s2.0-S037811191930（1727KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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