Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms | |
| Chen, Zhihang1,2,3; Wang, Xiuliang1,2; Li, Shuang1,2,3; Yao, Jianting1,2; Shao, Zhanru1,2; Duan, Delin1,2 | |
| 2019-08-02 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| ISSN | 1661-6596 |
| 卷号 | 20期号:16页码:14 |
| 通讯作者 | Wang, Xiuliang(xlwang@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn) |
| 摘要 | Tic20 is an important translocon protein that plays a role in protein transport in the chloroplast. The sequence of Tic20 was determined in the lower brown alga Saccharina japonica. Structural analysis of SjTic20 revealed a noncanonical structure consisting of an N-terminal non-cyanobacterium-originated EF-hand domain (a helix-loop-helix structural domain) and a C-terminal cyanobacterium-originated Tic20 domain. Subcellular localization and transmembrane analysis indicated that SjTic20 featured an M-type N-in-C-in-terminal orientation, with four transmembrane domains in the innermost membrane of the chloroplast in the microalga Phaeodactylum tricornutum, and the EF-hand domain was entirely extruded into the chloroplast stroma. Our study provides information on the structure, localization, and topological features of SjTic20, and further functional analysis of SjTic20 in S. japonica is needed. |
| 关键词 | Tic20 EF-hand Phaeodactylum tricornutum Saccharina japonica subcellular localization transmembrane topology |
| DOI | 10.3390/ijms20164000 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Nature Science Foundation of China[31772848] ; National Nature Science Foundation of China[31272660] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; [2017YFE0130900] ; National Nature Science Foundation of China[31772848] ; National Nature Science Foundation of China[31272660] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; [2017YFE0130900] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| WOS记录号 | WOS:000484411100157 |
| 出版者 | MDPI |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/162769 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Xiuliang; Duan, Delin |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100093, Peoples R China |
| 第一作者单位 | 中国科学院海洋大科学研究中心 |
| 通讯作者单位 | 中国科学院海洋大科学研究中心 |
| 推荐引用方式 GB/T 7714 | Chen, Zhihang,Wang, Xiuliang,Li, Shuang,et al. Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(16):14. |
| APA | Chen, Zhihang,Wang, Xiuliang,Li, Shuang,Yao, Jianting,Shao, Zhanru,&Duan, Delin.(2019).Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(16),14. |
| MLA | Chen, Zhihang,et al."Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.16(2019):14. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| 174-吴莹.pdf（2247KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
| Verification of the （5219KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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