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Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms
Chen, Zhihang1,2,3; Wang, Xiuliang1,2; Li, Shuang1,2,3; Yao, Jianting1,2; Shao, Zhanru1,2; Duan, Delin1,2
2019-08-02
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN1661-6596
Volume20Issue:16Pages:14
Corresponding AuthorWang, Xiuliang(xlwang@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn)
AbstractTic20 is an important translocon protein that plays a role in protein transport in the chloroplast. The sequence of Tic20 was determined in the lower brown alga Saccharina japonica. Structural analysis of SjTic20 revealed a noncanonical structure consisting of an N-terminal non-cyanobacterium-originated EF-hand domain (a helix-loop-helix structural domain) and a C-terminal cyanobacterium-originated Tic20 domain. Subcellular localization and transmembrane analysis indicated that SjTic20 featured an M-type N-in-C-in-terminal orientation, with four transmembrane domains in the innermost membrane of the chloroplast in the microalga Phaeodactylum tricornutum, and the EF-hand domain was entirely extruded into the chloroplast stroma. Our study provides information on the structure, localization, and topological features of SjTic20, and further functional analysis of SjTic20 in S. japonica is needed.
KeywordTic20 EF-hand Phaeodactylum tricornutum Saccharina japonica subcellular localization transmembrane topology
DOI10.3390/ijms20164000
Indexed BySCI
Language英语
Funding ProjectNational Nature Science Foundation of China[31772848] ; National Nature Science Foundation of China[31272660] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0502-1] ; [2017YFE0130900]
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS SubjectBiochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS IDWOS:000484411100157
PublisherMDPI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162769
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Xiuliang; Duan, Delin
Affiliation1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100093, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Zhihang,Wang, Xiuliang,Li, Shuang,et al. Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(16):14.
APA Chen, Zhihang,Wang, Xiuliang,Li, Shuang,Yao, Jianting,Shao, Zhanru,&Duan, Delin.(2019).Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(16),14.
MLA Chen, Zhihang,et al."Verification of the Saccharina japonica Translocon Tic20 and its Localization in the Chloroplast Membrane in Diatoms".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.16(2019):14.
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