IOCAS-IR  > 实验海洋生物学重点实验室
Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins
Zhao, Ping1,2,3,4; Niu, Jianfeng1,2,4; Huan, Li1,2,4; Gu, Wenhui1,2,4; Wu, Mingjiang5; Wang, Guangce1,2,4
2019-08-01
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
Volume31Issue:4Pages:2497-2505
Corresponding AuthorWang, Guangce(gcwang@qdio.ac.cn)
AbstractPyropia haitanensis is one of the most important and commercial species of edible seaweed in China. However, at its late growth stages it is unsuitable for food processing and is usually treated as waste. The present study investigated the full use of aging Py. haitanensis and attempted to improve its economic value through the purification of R-phycoerythrin combining the extraction of agar from the dry residue after phycobiliprotein extraction. The recovery of R-phycoerythrin from crude extract was 12.12% with a purification ratio of > 4. The agar yield was 5.81% with a gel strength of 796.00 +/- 28.10 g cm(-2), a gelling temperature of 38.10 +/- 0.22 degrees C, a melting temperature of 89.50 +/- 0.55 degrees C, 3,6-anhydrogalactose content of 34% +/- 3.16%, and sulfate content of 3.01% +/- 0.24%. There were no significant differences between these parameters and those from the direct extraction of Py. haitanensis. The agar extracted exhibited some physicochemical properties that have industrial applications. It could also be used as a material for DNA electrophoresis and a bacterial culture medium. This new method of comprehensive extraction reduced use of water and alkali, and waste liquid output, which were 36.52 L, 0.40 L, and 39.15 L, respectively, less than that from direct Py. haitanensis extraction. Total nitrogen, suspended solids, phosphorus, ammonia-nitrogen, chemical oxygen demand, and biochemical oxygen demand of the extract after 5 days were 38.37 mg, 4.72 x 10(3) mg, 62.82 mg, 18.32 mg, 8.46 x 10(3) mg, and 2.21 x 10(3) mg, respectively, less than that directly from Py. haitanensis.
KeywordPyropia haitanensis Expanded bed adsorption R-phycoerythrin Agar
DOI10.1007/s10811-019-1735-z
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2016YFC1400606] ; Natural Science Foundation of China[31470430] ; Natural Science Foundation of China[41876197]
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000479057200030
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/162364
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Guangce
Affiliation1.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
5.Wenzhou Univ, Coll Life & Environm Sci, Wenzhou, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhao, Ping,Niu, Jianfeng,Huan, Li,et al. Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins[J]. JOURNAL OF APPLIED PHYCOLOGY,2019,31(4):2497-2505.
APA Zhao, Ping,Niu, Jianfeng,Huan, Li,Gu, Wenhui,Wu, Mingjiang,&Wang, Guangce.(2019).Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins.JOURNAL OF APPLIED PHYCOLOGY,31(4),2497-2505.
MLA Zhao, Ping,et al."Agar extraction from Pyropia haitanensis residue after the removal and purification of phycobiliproteins".JOURNAL OF APPLIED PHYCOLOGY 31.4(2019):2497-2505.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao, Ping]'s Articles
[Niu, Jianfeng]'s Articles
[Huan, Li]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao, Ping]'s Articles
[Niu, Jianfeng]'s Articles
[Huan, Li]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao, Ping]'s Articles
[Niu, Jianfeng]'s Articles
[Huan, Li]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.