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Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae
Wu, Lele1,2,3,4; Han, Mingming1,2,3; Song, Zongcheng5; Xu, Shihong1,2,3; Li, Jun1,2,3; Li, Xueqing1; Wang, Yanfeng1,2,3; Yue, Xinlu5; Li, Xian1,2,3
2019-07-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume90Pages:328-337
Corresponding AuthorLi, Jun(junli@qclio.ac.cn) ; Li, Xueqing(lixian@qdio.ac.cn)
AbstractLight is a key environmental factor that synchronizes various life stages from embryo development to sexual maturation in fish. For turbot, light spectra have the most influence at the larval and juvenile stages. In the current study, differences in the development of embryos and the performance of newly hatched turbot larvae exposed to five different spectra: full spectrum (LDF), blue (LOB, peak at 450 nm), green (LDG, peak at 533 nm), orange (LDO, peak at 595 nm) and red (LDR, peak at 629 nm), were examined. At 62.8 h post fertilization, a higher number of embryos exposed to short-wavelengths (LDG and LOB) had developed a heartbeat in comparison with embryos exposed to other wavelengths. Larvae exposed to the green spectrum had higher malformation rates than larvae exposed to the other spectra, indicating that larvae exposed to green light may have significantly reduced survival rates. The results of non-specific immunity parameters showed that the mRNA expression levels of cathepsin D (CTSD), cathepsin F (CTSF), catalase (CAT) and metallothionein (MT) in larvae exposed to LDB were significantly higher than those exposed to other spectra, but CAT activity in larvae exposed to LDB was significantly lower than larvae exposed to the other spectra. There was no significant difference in MT activity in larvae exposed to the five different spectra. The mRNA expression level of lysozyme (LZM) in larvae exposed to LDR was significantly higher than other spectra, while there was no significant difference in LZM activity observed in larvae exposed to LDR, LDG, LDB and LDF. The difference of the enzyme activity of total superoxide dismutase (T-SOD) was not significant among larvae exposed to the five spectra. mRNA expression of the heat shock protein 70 (HSP70) was significantly higher in newly hatched larvae exposed to LDB, LDR and LDG, indicating that larvae exposed to LDB, LDG and LDR exhibited a stress response. The mRNA expression level of the insulin-like growth factor-1 (IGF-1) and growth parameters in the newly hatched larvae exposed to the different spectra were not significantly different. The results of the present study indicate that LDO and LDF should be used for embryo incubation and newly hatched larvae when rearing turbot. This study provides a theoretical basis for optimizing the incubation light environment for fertilized turbot eggs, promoting immunity and reducing stress responses in newly hatched larvae.
KeywordScophthalmus maximus Light spectrum Embryo Newly hatched larvae
DOI10.1016/j.fsi.2019.05.007
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2017YFB0404000] ; Key R&D Program of Shandong Province[2018GHY115031] ; Scientific and Technologic Development Program of Weihai[2017GNS05] ; China Agriculture Research System[CARS-47]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000471085000036
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161781
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Jun; Li, Xueqing
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Weihai Shenghang Aquat Prod Sci & Technol Co Ltd, Weihai 264200, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wu, Lele,Han, Mingming,Song, Zongcheng,et al. Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae[J]. FISH & SHELLFISH IMMUNOLOGY,2019,90:328-337.
APA Wu, Lele.,Han, Mingming.,Song, Zongcheng.,Xu, Shihong.,Li, Jun.,...&Li, Xian.(2019).Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae.FISH & SHELLFISH IMMUNOLOGY,90,328-337.
MLA Wu, Lele,et al."Effects of different light spectra on embryo development and the performance of newly hatched turbot (Scophthalmus maximus) larvae".FISH & SHELLFISH IMMUNOLOGY 90(2019):328-337.
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