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Two alpha-2 macroglobulin from Portunus trituberculatus involved in the prophenoloxidase system, phagocytosis and regulation of antimicrobial peptides
Ning, Junhao1,3,4; Liu, Yuan1,2,3; Gao, Fengtao1,2,3; Song, Chengwen1,2,3; Cui, Zhaoxia2,5
2019-06-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume89Pages:574-585
Corresponding AuthorCui, Zhaoxia(cuizhaoxia@nbu.edu.cn)
AbstractAlpha-2 macroglobulin (A2M) is a ubiquitous protease inhibitor involved in the innate host defense system. Herein, two distinct A2M genes (designated as PtA2M-1 and PtA2M-2, respectively) were isolated from the swimming crab Portunus trituberculatus. PtA2M-1 and PtA2M-2 encoded proteins with 1541 or 1516 amino acids, respectively, containing the typically functional domains of A2M. Unlike highly expressed in hemocytes of most arthropods, PtA2M-1 and PtA2M-2 were predominantly detected in gill, eyestalk and digestive tracks. During the embryonic stages, PtA2Ms were found to be expressed most highly in fertilized eggs, suggesting their maternal origin. After challenged with Vibrio alginolyticus, the transcripts of PtA2Ms showed similar time-dependent response expression pattern, while PtA2M-1 was more sensitive to Micrococcus luteus and Pichia pastoris infection than PtA2M-2. Knockdown of PtA2M-1 or PtA2M-2 could significantly enhance the expression of propheno-loxidase (proPO) associated genes (PtproPO and PtPPAF) and serine protease related genes (PtcSP1-3 and PtSPH), however, PtLSZ and the phagocytosis-related genes (PtMyosin and PtRab5) were effectively inhibited. These results were further supported by the PO and lysozyme activities in hemolymph of the PtA2M-1- or PtA2M-2-silenced crabs. In addition, PtA2M-1 and PtA2M-2 could regulate the expression of antimicrobial peptide (AMP) genes (PtALF1-3, PtCrustin1 and PtCrustin3) through the Toll and NE-kappa B pathways. Our findings together suggest that PtA2Ms might function in crab host defense via regulating the proPO system, phagocytosis and the expression of AMP genes.
KeywordPortunus trituberculatus Alpha-2 macroglobulin Expression profiles Phagocytosis Prophenoloxidase system AMP
DOI10.1016/j.fsi.2019.04.033
Indexed BySCI
Language英语
Funding ProjectNational Key R&D Program of China[2018YFD0900303] ; National Natural Science Foundation of China of China[41776159] ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02] ; Natural Science Foundation of Shandong Province[ZR2017QD001]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000469897700064
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161653
Collection实验海洋生物学重点实验室
Corresponding AuthorCui, Zhaoxia
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Ning, Junhao,Liu, Yuan,Gao, Fengtao,et al. Two alpha-2 macroglobulin from Portunus trituberculatus involved in the prophenoloxidase system, phagocytosis and regulation of antimicrobial peptides[J]. FISH & SHELLFISH IMMUNOLOGY,2019,89:574-585.
APA Ning, Junhao,Liu, Yuan,Gao, Fengtao,Song, Chengwen,&Cui, Zhaoxia.(2019).Two alpha-2 macroglobulin from Portunus trituberculatus involved in the prophenoloxidase system, phagocytosis and regulation of antimicrobial peptides.FISH & SHELLFISH IMMUNOLOGY,89,574-585.
MLA Ning, Junhao,et al."Two alpha-2 macroglobulin from Portunus trituberculatus involved in the prophenoloxidase system, phagocytosis and regulation of antimicrobial peptides".FISH & SHELLFISH IMMUNOLOGY 89(2019):574-585.
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