A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent

doi:10.3389/fcimb.2019.00183

IOCAS-IR > 实验海洋生物学重点实验室

	A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent
	Gu, Han-Jie 1,2,3; Sun, Qing-Lei1,2 ; Luo, Jing-Chang 1,2,3; Zhang, Jian 1,2; Sun, Li 1,2
	2019-05-31
发表期刊	FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN	2235-2988
卷号	9 页码:14
通讯作者	Sun, Li(lsun@qdio.ac.cn)
摘要	Bacillus subtilis is the best studied Gram-positive bacterium, primarily as a model of cell differentiation and industrial exploitation. To date, little is known about the virulence of B. subtilis. In this study, we examined the virulence potential of a B. subtilis strain (G7) isolated from the Iheya North hydrothermal field of Okinawa Trough. G7 is aerobic, motile, endospore-forming, and requires NaCl for growth. The genome of G7 is composed of one circular chromosome of 4,216,133 base pairs with an average GC content of 43.72%. G7 contains 4,416 coding genes, 27.5% of which could not be annotated, and the remaining 72.5% were annotated with known or predicted functions in 25 different COG categories. Ten sets of 23S, 5S, and 16S ribosomal RNA operons, 86 tRNA and 14 sRNA genes, 50 tandem repeats, 41 mini-satellites, one microsatellite, and 42 transposons were identified in G7. Comparing to the genome of the B. subtilis wild type strain NCIB 3610(T), G7 genome contains many genomic translocations, inversions, and insertions, and twice the amount of genomic Islands (GIs), with 42.5% of GI genes encoding hypothetical proteins. G7 possesses abundant putative virulence genes associated with adhesion, invasion, dissemination, anti-phagocytosis, and intracellular survival. Experimental studies showed that G7 was able to cause mortality in fish and mice following intramuscular/intraperitoneal injection, resist the killing effect of serum complement, and replicate in mouse macrophages and fish peripheral blood leukocytes. Taken together, our study indicates that G7 is a B. subtilis isolate with unique genetic features and can be lethal to vertebrate animals once being introduced into the animals by artificial means. These results provide the first insight into the potential harmfulness of deep-sea B. subtilis.
关键词	Bacillus subtilis deep-sea hydrothermal vent virulence genome
DOI	10.3389/fcimb.2019.00183
收录类别	SCI
语种	英语
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302] ; Taishan Scholar Program of Shandong Province ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Immunology ; Microbiology
WOS类目	Immunology ; Microbiology
WOS记录号	WOS:000470200100002
出版者	FRONTIERS MEDIA SA
引用统计	被引频次：29[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161599
专题	实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Gu, Han-Jie,Sun, Qing-Lei,Luo, Jing-Chang,et al. A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2019,9:14.
APA	Gu, Han-Jie,Sun, Qing-Lei,Luo, Jing-Chang,Zhang, Jian,&Sun, Li.(2019).A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,9,14.
MLA	Gu, Han-Jie,et al."A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 9(2019):14.

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