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A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent
Gu, Han-Jie1,2,3; Sun, Qing-Lei1,2; Luo, Jing-Chang1,2,3; Zhang, Jian1,2; Sun, Li1,2
2019-05-31
Source PublicationFRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN2235-2988
Volume9Pages:14
Corresponding AuthorSun, Li(lsun@qdio.ac.cn)
AbstractBacillus subtilis is the best studied Gram-positive bacterium, primarily as a model of cell differentiation and industrial exploitation. To date, little is known about the virulence of B. subtilis. In this study, we examined the virulence potential of a B. subtilis strain (G7) isolated from the Iheya North hydrothermal field of Okinawa Trough. G7 is aerobic, motile, endospore-forming, and requires NaCl for growth. The genome of G7 is composed of one circular chromosome of 4,216,133 base pairs with an average GC content of 43.72%. G7 contains 4,416 coding genes, 27.5% of which could not be annotated, and the remaining 72.5% were annotated with known or predicted functions in 25 different COG categories. Ten sets of 23S, 5S, and 16S ribosomal RNA operons, 86 tRNA and 14 sRNA genes, 50 tandem repeats, 41 mini-satellites, one microsatellite, and 42 transposons were identified in G7. Comparing to the genome of the B. subtilis wild type strain NCIB 3610(T), G7 genome contains many genomic translocations, inversions, and insertions, and twice the amount of genomic Islands (GIs), with 42.5% of GI genes encoding hypothetical proteins. G7 possesses abundant putative virulence genes associated with adhesion, invasion, dissemination, anti-phagocytosis, and intracellular survival. Experimental studies showed that G7 was able to cause mortality in fish and mice following intramuscular/intraperitoneal injection, resist the killing effect of serum complement, and replicate in mouse macrophages and fish peripheral blood leukocytes. Taken together, our study indicates that G7 is a B. subtilis isolate with unique genetic features and can be lethal to vertebrate animals once being introduced into the animals by artificial means. These results provide the first insight into the potential harmfulness of deep-sea B. subtilis.
KeywordBacillus subtilis deep-sea hydrothermal vent virulence genome
DOI10.3389/fcimb.2019.00183
Indexed BySCI
Language英语
Funding ProjectStrategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302] ; Taishan Scholar Program of Shandong Province
WOS Research AreaImmunology ; Microbiology
WOS SubjectImmunology ; Microbiology
WOS IDWOS:000470200100002
PublisherFRONTIERS MEDIA SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/161599
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Li
Affiliation1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China
2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Gu, Han-Jie,Sun, Qing-Lei,Luo, Jing-Chang,et al. A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2019,9:14.
APA Gu, Han-Jie,Sun, Qing-Lei,Luo, Jing-Chang,Zhang, Jian,&Sun, Li.(2019).A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,9,14.
MLA Gu, Han-Jie,et al."A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 9(2019):14.
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