Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent | |
Gu, Han-Jie1,2,3; Sun, Qing-Lei1,2 ; Luo, Jing-Chang1,2,3; Zhang, Jian1,2; Sun, Li1,2
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| 2019-05-31 | |
| Source Publication | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
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| ISSN | 2235-2988 |
| Volume | 9Pages:14 |
| Corresponding Author | Sun, Li(lsun@qdio.ac.cn) |
| Abstract | Bacillus subtilis is the best studied Gram-positive bacterium, primarily as a model of cell differentiation and industrial exploitation. To date, little is known about the virulence of B. subtilis. In this study, we examined the virulence potential of a B. subtilis strain (G7) isolated from the Iheya North hydrothermal field of Okinawa Trough. G7 is aerobic, motile, endospore-forming, and requires NaCl for growth. The genome of G7 is composed of one circular chromosome of 4,216,133 base pairs with an average GC content of 43.72%. G7 contains 4,416 coding genes, 27.5% of which could not be annotated, and the remaining 72.5% were annotated with known or predicted functions in 25 different COG categories. Ten sets of 23S, 5S, and 16S ribosomal RNA operons, 86 tRNA and 14 sRNA genes, 50 tandem repeats, 41 mini-satellites, one microsatellite, and 42 transposons were identified in G7. Comparing to the genome of the B. subtilis wild type strain NCIB 3610(T), G7 genome contains many genomic translocations, inversions, and insertions, and twice the amount of genomic Islands (GIs), with 42.5% of GI genes encoding hypothetical proteins. G7 possesses abundant putative virulence genes associated with adhesion, invasion, dissemination, anti-phagocytosis, and intracellular survival. Experimental studies showed that G7 was able to cause mortality in fish and mice following intramuscular/intraperitoneal injection, resist the killing effect of serum complement, and replicate in mouse macrophages and fish peripheral blood leukocytes. Taken together, our study indicates that G7 is a B. subtilis isolate with unique genetic features and can be lethal to vertebrate animals once being introduced into the animals by artificial means. These results provide the first insight into the potential harmfulness of deep-sea B. subtilis. |
| Keyword | Bacillus subtilis deep-sea hydrothermal vent virulence genome |
| DOI | 10.3389/fcimb.2019.00183 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Shandong Major Science and Technology Innovation Project[2018SDKJ0302] ; Taishan Scholar Program of Shandong Province |
| WOS Research Area | Immunology ; Microbiology |
| WOS Subject | Immunology ; Microbiology |
| WOS ID | WOS:000470200100002 |
| Publisher | FRONTIERS MEDIA SA |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/161599 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sun, Li |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS Key Lab Expt Marine Biol, Qingdao, Shandong, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Gu, Han-Jie,Sun, Qing-Lei,Luo, Jing-Chang,et al. A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2019,9:14. |
| APA | Gu, Han-Jie,Sun, Qing-Lei,Luo, Jing-Chang,Zhang, Jian,&Sun, Li.(2019).A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,9,14. |
| MLA | Gu, Han-Jie,et al."A First Study of the Virulence Potential of a Bacillus subtilis Isolate From Deep-Sea Hydrothermal Vent".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 9(2019):14. |
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