Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum)

doi:10.1371/journal.pone.0215158

	Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum)
	Li, Qiao 1,2; Sun, Song1,2,3,4,5 ; Zhang, Fang 1,2,3,4; Wang, Minxiao 1,3,4; Li, Mengna 1,2
	2019-04-18
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	14 期号:4 页码:21
通讯作者	Sun, Song(sunsong@qdio.ac.cn)
摘要	The Manila clam Ruditapes philippinarum has become a common and dominant macrobenthic species in coastal areas of the northwestern Pacific and temperate waters of Europe; it is also a major cultured shellfish, with annual worldwide production exceeding 3.3 million tonnes. This species faces greater risk of exposure to hypoxia as eutrophication worsens throughout its coastal habitats; however, its tolerance to hypoxia remains unclear, and the toxicological indicators including LC50 and LT50 have not yet been assessed. Previous studies on the effects of hypoxia on marine benthos have focused largely on functional responses, such as metabolism and gene expression, leaving potential structural damage to the mitochondria or the cells unknown. In this study we assessed the effects of hypoxia on Manila clam in terms of survival, behavior, metabolism and cellular damage, using a newly designed automated hypoxia simulation device that features exceptional accuracy and good stability. The clams exhibited strong tolerance to hypoxia as the 20-day LC50 for dissolved oxygen (DO) was estimated to be 0.57 mg L-1, and the LT50 at 0.5 mg L-1 DO was 422 hours. Adaptations included fewer buried clams and a depressed metabolism, while the unexpected rise in the activities of key enzymes involved in glycolysis may indicate a diverse strategy of shellfish under hypoxia. Cellular damage was observed as collapse of the mitochondrial cristae and both cellular and mitochondrial vacuolization. This multi-level study complements and updates our knowledge of the effects of hypoxia on marine benthos, by improving our understanding of the potential for marine ecological transformation under hypoxic conditions and providing useful information for Manila clam farming.
DOI	10.1371/journal.pone.0215158
收录类别	SCI
语种	英语
资助项目	NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA19060201] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050501] ; National Key R&D Program of China[2017YFC1404405] ; Taishan Scholars Project ; NSFC-Shandong Joint Fund for Marine Ecology and Environmental Sciences[U1606404] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA19060201] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA23050501] ; National Key R&D Program of China[2017YFC1404405] ; Taishan Scholars Project
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000465015500025
出版者	PUBLIC LIBRARY SCIENCE
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/161253
专题	海洋生态与环境科学重点实验室
通讯作者	Sun, Song
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Shandong, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Shandong, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Jiaozhou Bay Marine Ecosyst Res Stn, Qingdao, Shandong, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Li, Qiao,Sun, Song,Zhang, Fang,et al. Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum)[J]. PLOS ONE,2019,14(4):21.
APA	Li, Qiao,Sun, Song,Zhang, Fang,Wang, Minxiao,&Li, Mengna.(2019).Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum).PLOS ONE,14(4),21.
MLA	Li, Qiao,et al."Effects of hypoxia on survival, behavior, metabolism and cellular damage of Manila clam (Ruditapes philippinarum)".PLOS ONE 14.4(2019):21.

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