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Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method
Li, Qiuyu1,2,3,4; Zhao, Weihong1,2,3,4; Miao, Hui1,2,3; Han, Xiaotian1,2,3
2019-02-01
Source PublicationMARINE POLLUTION BULLETIN
ISSN0025-326X
Volume139Pages:136-140
Corresponding AuthorZhao, Weihong(whzhao@qdio.ac.cn)
AbstractThis work attempted to prolong the validity of the molybdate mixed solution and ascorbic acid solution used in the phosphomolybdenum blue spectrophotometric method by improving their preservation according to the influence factors. The results showed that the molybdate mixed solution can be directly preserved in darkness with validity over half a year. The ascorbic acid solution is influenced by light, temperature, pH, metal ions, oxygen, and bacteria. The validity of ascorbic acid is shortened as the temperature rises. Through keeping in darkness, adding complexing agents, adjusting pH, removing oxygen and sterilization, the validity of ascorbic acid solution was prolonged to over 2.7 times under 4 degrees C and over 5 times under 25 degrees C. At the same time, the hybrid solution of ascorbic acid solution and molybdate mixed solution should be preserved separately, otherwise the using effect is poor.
KeywordMarine phosphate Marine phosphate detection Phosphomolybdenum blue spectrophotometric method Reagents preservation
DOI10.1016/j.marpolbul.2018.12.011
Indexed BySCI
Language英语
Funding ProjectOpen Financial Grant from Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0410] ; Joint Funds of the National Natural Science Foundation of China[U1606404] ; State Oceanic Administration, People's Republic of China
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology
WOS SubjectEnvironmental Sciences ; Marine & Freshwater Biology
WOS IDWOS:000458227800017
PublisherPERGAMON-ELSEVIER SCIENCE LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160844
Collection海洋生态与环境科学重点实验室
Corresponding AuthorZhao, Weihong
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Beijing, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Qiuyu,Zhao, Weihong,Miao, Hui,et al. Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method[J]. MARINE POLLUTION BULLETIN,2019,139:136-140.
APA Li, Qiuyu,Zhao, Weihong,Miao, Hui,&Han, Xiaotian.(2019).Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method.MARINE POLLUTION BULLETIN,139,136-140.
MLA Li, Qiuyu,et al."Effects and improvements of different reagents preservation methods on the determination of phosphate in seawater by phosphomolybdenum blue spectrophotometric method".MARINE POLLUTION BULLETIN 139(2019):136-140.
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