Knowledge Management System Of Institute of Oceanology, Chinese Academy of Sciences
| The ethanol extract of honeysuckle stem modulates the innate immunity of Chinese mitten crab Eriocheir sinensis against Aeromonas hydrophila | |
Zhao, Zhilong1; Zhang, Haijuan1; Wang, Mengqiang2 ; Zhang, Chun1; Kuang, Pengqun1; Zhou, Zhi3; Zhang, Guizhi1; Wang, Zhen1; Zhang, Bianbian1; Shi, Xiaowei1
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| 2018-11-01 | |
| 发表期刊 | FISH & SHELLFISH IMMUNOLOGY
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| ISSN | 1050-4648 |
| 卷号 | 82页码:304-311 |
| 通讯作者 | Wang, Zhen(wangzhen@lyu.edu.cn) ; Shi, Xiaowei(shixiaowei@lyu.edu.cn) |
| 摘要 | Honeysuckle stem had been used as feed additives to modulate immunity in breeding industry, which was limited in the aquaculture field. In this study, the immunomodulation of honeysuckle stem ethanol extract (designed as HSE) on Chinese mitten crab Eriocheir sinensis was detected. The crabs fed with HSE diets for 30 days had higher level of the total haemocyte count (HTC), lysozyme activity and PO activity (P < 0.05), and had no obvious affect on the phagocytic activity, NO and TNF-alpha level. When challenged with Aeromonas hydrophila (1.0 x 10(7) colony-forming units), HSE exhibited weak antibacterial activity against A. hydrophila and increased survival rate of crabs. The decreasing of THC and the increasing of TNF-alpha concentration, EsCaspase and EsLITAF mRNA expression level were all inhibited significantly by HSE treatment (P < 0.05), when the crabs were challenged by A. hydrophila. Moreover, the following immune parameters of crabs were enhanced by HSE treatment after A. hydrophila infection, including the rising of phagocytosis index and phagocytic rate of haemocyte, the rising of lysozyme, PO, NOS activities and nitric oxide concentration (P < 0.05). Therefore, it was concluded that HSE had great potential to develop into feed additive of crabs, which could enhance the innate immunity of Chinese mitten crabs E. sinensis effectively after A. hydrophila infection. |
| 关键词 | Honeysuckle stem Eriocheir sinensis Aeromonas hydrophila Immunomodulation |
| DOI | 10.1016/j.fsi.2018.08.035 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | China Postdoctoral Science Foundation[2017M610427] ; Shandong Natural Science Foundation Project[ZR2016CP22] ; National Natural Science Foundation of China[31300545] ; National Natural Science Foundation of China[31700238] ; Key R & D project in Shandong[2017YYSP027] ; Shandong Province Higher Educational Science and Technology Program[J15LM11] ; Shandong Provincial Natural Science Foundation of China[ZR2017PC007] ; China Postdoctoral Science Foundation[2017M610427] ; Shandong Natural Science Foundation Project[ZR2016CP22] ; National Natural Science Foundation of China[31300545] ; National Natural Science Foundation of China[31700238] ; Key R & D project in Shandong[2017YYSP027] ; Shandong Province Higher Educational Science and Technology Program[J15LM11] ; Shandong Provincial Natural Science Foundation of China[ZR2017PC007] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS类目 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| WOS记录号 | WOS:000447571600036 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/160483 |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Wang, Zhen; Shi, Xiaowei |
| 作者单位 | 1.Linyi Univ, Coll Pharm, Linyi 276000, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 3.Hainan Univ, Minist Educ, Key Lab Trop Biol Resources, Haikou 570228, Hainan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Zhilong,Zhang, Haijuan,Wang, Mengqiang,et al. The ethanol extract of honeysuckle stem modulates the innate immunity of Chinese mitten crab Eriocheir sinensis against Aeromonas hydrophila[J]. FISH & SHELLFISH IMMUNOLOGY,2018,82:304-311. |
| APA | Zhao, Zhilong.,Zhang, Haijuan.,Wang, Mengqiang.,Zhang, Chun.,Kuang, Pengqun.,...&Shi, Xiaowei.(2018).The ethanol extract of honeysuckle stem modulates the innate immunity of Chinese mitten crab Eriocheir sinensis against Aeromonas hydrophila.FISH & SHELLFISH IMMUNOLOGY,82,304-311. |
| MLA | Zhao, Zhilong,et al."The ethanol extract of honeysuckle stem modulates the innate immunity of Chinese mitten crab Eriocheir sinensis against Aeromonas hydrophila".FISH & SHELLFISH IMMUNOLOGY 82(2018):304-311. |
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| The ethanol extract （9130KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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