IOCAS-IR
The ethanol extract of honeysuckle stem modulates the innate immunity of Chinese mitten crab Eriocheir sinensis against Aeromonas hydrophila
Zhao, Zhilong1; Zhang, Haijuan1; Wang, Mengqiang2; Zhang, Chun1; Kuang, Pengqun1; Zhou, Zhi3; Zhang, Guizhi1; Wang, Zhen1; Zhang, Bianbian1; Shi, Xiaowei1
2018-11-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume82Pages:304-311
Corresponding AuthorWang, Zhen(wangzhen@lyu.edu.cn) ; Shi, Xiaowei(shixiaowei@lyu.edu.cn)
AbstractHoneysuckle stem had been used as feed additives to modulate immunity in breeding industry, which was limited in the aquaculture field. In this study, the immunomodulation of honeysuckle stem ethanol extract (designed as HSE) on Chinese mitten crab Eriocheir sinensis was detected. The crabs fed with HSE diets for 30 days had higher level of the total haemocyte count (HTC), lysozyme activity and PO activity (P < 0.05), and had no obvious affect on the phagocytic activity, NO and TNF-alpha level. When challenged with Aeromonas hydrophila (1.0 x 10(7) colony-forming units), HSE exhibited weak antibacterial activity against A. hydrophila and increased survival rate of crabs. The decreasing of THC and the increasing of TNF-alpha concentration, EsCaspase and EsLITAF mRNA expression level were all inhibited significantly by HSE treatment (P < 0.05), when the crabs were challenged by A. hydrophila. Moreover, the following immune parameters of crabs were enhanced by HSE treatment after A. hydrophila infection, including the rising of phagocytosis index and phagocytic rate of haemocyte, the rising of lysozyme, PO, NOS activities and nitric oxide concentration (P < 0.05). Therefore, it was concluded that HSE had great potential to develop into feed additive of crabs, which could enhance the innate immunity of Chinese mitten crabs E. sinensis effectively after A. hydrophila infection.
KeywordHoneysuckle stem Eriocheir sinensis Aeromonas hydrophila Immunomodulation
DOI10.1016/j.fsi.2018.08.035
Indexed BySCI
Language英语
Funding ProjectChina Postdoctoral Science Foundation[2017M610427] ; Shandong Natural Science Foundation Project[ZR2016CP22] ; National Natural Science Foundation of China[31300545] ; National Natural Science Foundation of China[31700238] ; Key R & D project in Shandong[2017YYSP027] ; Shandong Province Higher Educational Science and Technology Program[J15LM11] ; Shandong Provincial Natural Science Foundation of China[ZR2017PC007]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000447571600036
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160483
Collection中国科学院海洋研究所
Corresponding AuthorWang, Zhen; Shi, Xiaowei
Affiliation1.Linyi Univ, Coll Pharm, Linyi 276000, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Hainan Univ, Minist Educ, Key Lab Trop Biol Resources, Haikou 570228, Hainan, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Zhilong,Zhang, Haijuan,Wang, Mengqiang,et al. The ethanol extract of honeysuckle stem modulates the innate immunity of Chinese mitten crab Eriocheir sinensis against Aeromonas hydrophila[J]. FISH & SHELLFISH IMMUNOLOGY,2018,82:304-311.
APA Zhao, Zhilong.,Zhang, Haijuan.,Wang, Mengqiang.,Zhang, Chun.,Kuang, Pengqun.,...&Shi, Xiaowei.(2018).The ethanol extract of honeysuckle stem modulates the innate immunity of Chinese mitten crab Eriocheir sinensis against Aeromonas hydrophila.FISH & SHELLFISH IMMUNOLOGY,82,304-311.
MLA Zhao, Zhilong,et al."The ethanol extract of honeysuckle stem modulates the innate immunity of Chinese mitten crab Eriocheir sinensis against Aeromonas hydrophila".FISH & SHELLFISH IMMUNOLOGY 82(2018):304-311.
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