Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei

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	Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei
	Chen, Y.1,2,3 ; Wang, B. J.1,2; Wang, M. Q.1,2; Liu, M.1,2; Jiang, K. Y.1,2; Wang, L.1,2
	2018
发表期刊	ISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN	1824-307X
卷号	15 页码:316-326
通讯作者	Wang, L.(wanglei@qdio.ac.cn)
摘要	The reactive oxygen species (ROS) generated by dual oxidases (Duox) play a role in innate immunity in many organisms. In this study, a 4,735 bp full-length cDNA of the Pacific white shrimp dual oxidase (LvDuox) gene was cloned; the sequence included an open reading frame of 4,497 bp, encoding a protein of 1,498 as with a calculated mass of 173 kDa. Structural analysis revealed that LvDuox contains several domains. Homology analysis revealed that LvDuox exhibits 96.1%, 67.3% and 67.3% sequence identity with Marsupenaeus japonicas, Drosophila melanogaster and Acyrthosiphon pisum Duox, respectively. The mRNA transcripts of LvDuox were detected in all tested tissues. The mRNA expression of LvDuox was significantly induced in the midgut after Vibrio parahaemolyticus E1 (VPE1) stimulation. After the level of H2O2 in the midgut increased, expression of the superoxide dismutase and catalase genes in the midgut increased significantly. These results suggested that the LvDuox gene was upregulated in the midgut after the challenge by VPE1, and antioxidant genes were involved in the regulation of ROS in the shrimp midgut. LvDuox may therefore be a new target for intestinal disease research in the Pacific white shrimp.
关键词	Litopenaeus vannamei Duox antioxidant gene innate immunity
收录类别	SCI
语种	英语
资助项目	Science and Technology Development Fund Project of Shinan District of Qingdao City[2018-4-001-ZH] ; Chinese Academy of Sciences STS Regional Center Project (Fujian Province) ; Science and Technology Development Fund Project of Shinan District of Qingdao City[2018-4-001-ZH] ; Chinese Academy of Sciences STS Regional Center Project (Fujian Province)
WOS研究方向	Immunology ; Zoology
WOS类目	Immunology ; Zoology
WOS记录号	WOS:000445344500002
出版者	INVERTEBRATE SURVIVAL JOURNAL
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/160203
专题	实验海洋生物学重点实验室
通讯作者	Wang, L.
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Chen, Y.,Wang, B. J.,Wang, M. Q.,et al. Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:316-326.
APA	Chen, Y.,Wang, B. J.,Wang, M. Q.,Liu, M.,Jiang, K. Y.,&Wang, L..(2018).Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,316-326.
MLA	Chen, Y.,et al."Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):316-326.

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