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Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei
Chen, Y.1,2,3; Wang, B. J.1,2; Wang, M. Q.1,2; Liu, M.1,2; Jiang, K. Y.1,2; Wang, L.1,2
2018
Source PublicationISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN1824-307X
Volume15Pages:316-326
Corresponding AuthorWang, L.(wanglei@qdio.ac.cn)
AbstractThe reactive oxygen species (ROS) generated by dual oxidases (Duox) play a role in innate immunity in many organisms. In this study, a 4,735 bp full-length cDNA of the Pacific white shrimp dual oxidase (LvDuox) gene was cloned; the sequence included an open reading frame of 4,497 bp, encoding a protein of 1,498 as with a calculated mass of 173 kDa. Structural analysis revealed that LvDuox contains several domains. Homology analysis revealed that LvDuox exhibits 96.1%, 67.3% and 67.3% sequence identity with Marsupenaeus japonicas, Drosophila melanogaster and Acyrthosiphon pisum Duox, respectively. The mRNA transcripts of LvDuox were detected in all tested tissues. The mRNA expression of LvDuox was significantly induced in the midgut after Vibrio parahaemolyticus E1 (VPE1) stimulation. After the level of H2O2 in the midgut increased, expression of the superoxide dismutase and catalase genes in the midgut increased significantly. These results suggested that the LvDuox gene was upregulated in the midgut after the challenge by VPE1, and antioxidant genes were involved in the regulation of ROS in the shrimp midgut. LvDuox may therefore be a new target for intestinal disease research in the Pacific white shrimp.
KeywordLitopenaeus vannamei Duox antioxidant gene innate immunity
Indexed BySCI
Language英语
Funding ProjectScience and Technology Development Fund Project of Shinan District of Qingdao City[2018-4-001-ZH] ; Chinese Academy of Sciences STS Regional Center Project (Fujian Province)
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000445344500002
PublisherINVERTEBRATE SURVIVAL JOURNAL
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160203
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, L.
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Chen, Y.,Wang, B. J.,Wang, M. Q.,et al. Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:316-326.
APA Chen, Y.,Wang, B. J.,Wang, M. Q.,Liu, M.,Jiang, K. Y.,&Wang, L..(2018).Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,316-326.
MLA Chen, Y.,et al."Molecular characterization of the dual oxidase (LvDuox) gene from the pacific white shrimp Litopenaeus vannamei".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):316-326.
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