Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri | |
Wang, M.1; Wang, L.3,4,5 ; Ni, D.1; Yi, Q.3,4,5; Wang, X.6; Jia, Z.1; Song, L.2,3,4,5
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| 2018 | |
| Source Publication | ISJ-INVERTEBRATE SURVIVAL JOURNAL
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| ISSN | 1824-307X |
| Volume | 15Pages:302-315 |
| Corresponding Author | Song, L.(lshsong@dlou.edu.cn) |
| Abstract | Glutathione S-transferase (GST) is a superfamily of multifunction enzymes with varying catalytic roles in cellular detoxification to protect hosts against oxidative damage. In the present study, six GST genes were identified from Chlamys farreri, including CfGST omega, CfGST sigma-1, CfGST sigma-2, CfGST rho, CfGST zeta and CfmGST. CfGSTs shared high similarities with their counterparts from other species, and were clustered with their homologues into the corresponding clades in the phylogenetic tree, respectively. We investigated the distribution of their mRNA transcripts in different tissues and their temporal expression profiles in hemocytes after microbe stimulations by quantitative real-time PCR. The six CfGST genes were detectable in all the tested tissues, including hemocytes, muscle, mantle, gill, hepatopancreas, and gonad. Stimulations with various microbes drastically induced the mRNA transcripts of all the CfGSTs with different expression profiles. For examples, CfGST omega could be induced by three kinds of microbes, including Vibrio anguillarum, Micrococcus luteus and Pichia pastoris, whereas CfmGST could be only induced by V. anguillarum. These results indicated a powerful detoxification system of GSTs in scallop. Moreover, the distinct mRNA expression profiles of CfGSTs indicated their versatile and immune-challenge specific roles in the mollusk C. farreri. |
| Keyword | Chlamys farreri Glutathione S-transferase innate immunity |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[31530069] ; National Natural Science Foundation of China[31530069] |
| WOS Research Area | Immunology ; Zoology |
| WOS Subject | Immunology ; Zoology |
| WOS ID | WOS:000445344500001 |
| Publisher | INVERTEBRATE SURVIVAL JOURNAL |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/160200 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Song, L. |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China 4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 5.Dalian Ocean Univ, Dalian Key Lab Dis Prevent & Control Aquaculture, Dalian 116023, Peoples R China 6.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Wang, M.,Wang, L.,Ni, D.,et al. The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:302-315. |
| APA | Wang, M..,Wang, L..,Ni, D..,Yi, Q..,Wang, X..,...&Song, L..(2018).The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,302-315. |
| MLA | Wang, M.,et al."The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):302-315. |
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| The mRNA expression （1023KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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