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The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri
Wang, M.1; Wang, L.3,4,5; Ni, D.1; Yi, Q.3,4,5; Wang, X.6; Jia, Z.1; Song, L.2,3,4,5
2018
Source PublicationISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN1824-307X
Volume15Pages:302-315
Corresponding AuthorSong, L.(lshsong@dlou.edu.cn)
AbstractGlutathione S-transferase (GST) is a superfamily of multifunction enzymes with varying catalytic roles in cellular detoxification to protect hosts against oxidative damage. In the present study, six GST genes were identified from Chlamys farreri, including CfGST omega, CfGST sigma-1, CfGST sigma-2, CfGST rho, CfGST zeta and CfmGST. CfGSTs shared high similarities with their counterparts from other species, and were clustered with their homologues into the corresponding clades in the phylogenetic tree, respectively. We investigated the distribution of their mRNA transcripts in different tissues and their temporal expression profiles in hemocytes after microbe stimulations by quantitative real-time PCR. The six CfGST genes were detectable in all the tested tissues, including hemocytes, muscle, mantle, gill, hepatopancreas, and gonad. Stimulations with various microbes drastically induced the mRNA transcripts of all the CfGSTs with different expression profiles. For examples, CfGST omega could be induced by three kinds of microbes, including Vibrio anguillarum, Micrococcus luteus and Pichia pastoris, whereas CfmGST could be only induced by V. anguillarum. These results indicated a powerful detoxification system of GSTs in scallop. Moreover, the distinct mRNA expression profiles of CfGSTs indicated their versatile and immune-challenge specific roles in the mollusk C. farreri.
KeywordChlamys farreri Glutathione S-transferase innate immunity
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31530069]
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000445344500001
PublisherINVERTEBRATE SURVIVAL JOURNAL
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/160200
Collection实验海洋生物学重点实验室
Corresponding AuthorSong, L.
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
5.Dalian Ocean Univ, Dalian Key Lab Dis Prevent & Control Aquaculture, Dalian 116023, Peoples R China
6.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Wang, M.,Wang, L.,Ni, D.,et al. The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri[J]. ISJ-INVERTEBRATE SURVIVAL JOURNAL,2018,15:302-315.
APA Wang, M..,Wang, L..,Ni, D..,Yi, Q..,Wang, X..,...&Song, L..(2018).The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri.ISJ-INVERTEBRATE SURVIVAL JOURNAL,15,302-315.
MLA Wang, M.,et al."The mRNA expression profiles demonstrating versatile roles of glutathione S-transferase genes in the mollusk Chlamys farreri".ISJ-INVERTEBRATE SURVIVAL JOURNAL 15(2018):302-315.
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