Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis | |
Zhang, Shujing1,3; Wang, Hongxia1; Yu, Jiajia1,3; Jiang, Fengjuan1,3; Yue, Xin1; Liu, Baozhong1,2
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| 2018-11-01 | |
| Source Publication | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
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| ISSN | 1096-4959 |
| Volume | 225Pages:75-83 |
| Corresponding Author | Yue, Xin(yuexin@qdio.ac.cn) |
| Abstract | The microphthalmia-associated transcription factor (MITF) is a master regulator of melanocyte development through the direct transcriptional control of related genes, e.g., the phenoloxidase gene. In this study, an MITF gene, MpMITF2, was identified in the clam Meretrix petechialis. The full-length cDNA of MpMITF2 was 2026 bp, and the molecular mass of the predicted protein was 42.6 kDa. A basic helix-loop-helix leucine zipper domain was detected in the deduced protein sequence, which can bind the E-box motif within the promoter of the downstream genes. The mRNA of MpMITF2 was more highly expressed in the mantle compared to the other four tissues. Furthermore, there was a significant difference in the mRNA expression of MpMITF2 among three clam strains with different shell colors. The protein level of MpMITF2 was also different among these strains. These results implied that MpMITF2 was associated with shell color formation in the clam M. petechialis. When the mRNA expression of MpMITF2 was knocked down, the new shell showed discontinuous pigment distribution, suggesting that the reduced expression of MpMITF2 influenced pigment synthesis. A gene encoding phenoloxidase (MpPO) was identified as related to the shell color of the clam and was also a putative downstream gene of MITF. Both the mRNA and protein levels of MpPO decreased significantly at 12 h post-MpMITF-suppression, suggesting that MpMITF2 is required for the expression of MpPO. Our results indicate the close relationships among MpMITF2, MpPO and shell color. This study implicates the role of MITF in shell color formation in the clam M. petechialis. |
| Keyword | Meretrix petechialis MITF Phenoloxidase Shell color |
| DOI | 10.1016/j.cbpb.2018.04.007 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | China Agriculture Research System[CARS-49] ; National Natural Science Foundation of China[NSFC 31772845] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106] |
| WOS Research Area | Biochemistry & Molecular Biology ; Zoology |
| WOS Subject | Biochemistry & Molecular Biology ; Zoology |
| WOS ID | WOS:000442974200009 |
| Publisher | ELSEVIER SCIENCE INC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/159955 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Yue, Xin |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Zhang, Shujing,Wang, Hongxia,Yu, Jiajia,et al. Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2018,225:75-83. |
| APA | Zhang, Shujing,Wang, Hongxia,Yu, Jiajia,Jiang, Fengjuan,Yue, Xin,&Liu, Baozhong.(2018).Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,225,75-83. |
| MLA | Zhang, Shujing,et al."Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 225(2018):75-83. |
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| Identification of a （2452KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Application Full Text | |
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