IOCAS-IR  > 实验海洋生物学重点实验室
Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis
Zhang, Shujing1,3; Wang, Hongxia1; Yu, Jiajia1,3; Jiang, Fengjuan1,3; Yue, Xin1; Liu, Baozhong1,2
2018-11-01
Source PublicationCOMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
Volume225Pages:75-83
Corresponding AuthorYue, Xin(yuexin@qdio.ac.cn)
AbstractThe microphthalmia-associated transcription factor (MITF) is a master regulator of melanocyte development through the direct transcriptional control of related genes, e.g., the phenoloxidase gene. In this study, an MITF gene, MpMITF2, was identified in the clam Meretrix petechialis. The full-length cDNA of MpMITF2 was 2026 bp, and the molecular mass of the predicted protein was 42.6 kDa. A basic helix-loop-helix leucine zipper domain was detected in the deduced protein sequence, which can bind the E-box motif within the promoter of the downstream genes. The mRNA of MpMITF2 was more highly expressed in the mantle compared to the other four tissues. Furthermore, there was a significant difference in the mRNA expression of MpMITF2 among three clam strains with different shell colors. The protein level of MpMITF2 was also different among these strains. These results implied that MpMITF2 was associated with shell color formation in the clam M. petechialis. When the mRNA expression of MpMITF2 was knocked down, the new shell showed discontinuous pigment distribution, suggesting that the reduced expression of MpMITF2 influenced pigment synthesis. A gene encoding phenoloxidase (MpPO) was identified as related to the shell color of the clam and was also a putative downstream gene of MITF. Both the mRNA and protein levels of MpPO decreased significantly at 12 h post-MpMITF-suppression, suggesting that MpMITF2 is required for the expression of MpPO. Our results indicate the close relationships among MpMITF2, MpPO and shell color. This study implicates the role of MITF in shell color formation in the clam M. petechialis.
KeywordMeretrix petechialis MITF Phenoloxidase Shell color
DOI10.1016/j.cbpb.2018.04.007
Indexed BySCI
Language英语
Funding ProjectChina Agriculture Research System[CARS-49] ; National Natural Science Foundation of China[NSFC 31772845] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-106]
WOS Research AreaBiochemistry & Molecular Biology ; Zoology
WOS SubjectBiochemistry & Molecular Biology ; Zoology
WOS IDWOS:000442974200009
PublisherELSEVIER SCIENCE INC
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159955
Collection实验海洋生物学重点实验室
Corresponding AuthorYue, Xin
Affiliation1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, 1 Wenhai Rd, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zhang, Shujing,Wang, Hongxia,Yu, Jiajia,et al. Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2018,225:75-83.
APA Zhang, Shujing,Wang, Hongxia,Yu, Jiajia,Jiang, Fengjuan,Yue, Xin,&Liu, Baozhong.(2018).Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,225,75-83.
MLA Zhang, Shujing,et al."Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 225(2018):75-83.
Files in This Item:
File Name/Size DocType Version Access License
Identification of a (2452KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Shujing]'s Articles
[Wang, Hongxia]'s Articles
[Yu, Jiajia]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Shujing]'s Articles
[Wang, Hongxia]'s Articles
[Yu, Jiajia]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Shujing]'s Articles
[Wang, Hongxia]'s Articles
[Yu, Jiajia]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Identification of a gene encoding microphthalmia-associated transcription factor and its association with shell color in the clam Meretrix petechialis.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.