Penaeid shrimp brachyury: sequence analysis and expression during gastrulation

doi:10.1007/s00427-018-0618-7

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	Penaeid shrimp brachyury: sequence analysis and expression during gastrulation
	Hertzler, Philip L.1; Wei, Jiankai2 ; Droste, Andrew P.1; Yuan, Jianbo 3,4; Xiang, Jianhai3,4
	2018-09-01
发表期刊	DEVELOPMENT GENES AND EVOLUTION
ISSN	0949-944X
卷号	228 期号:5 页码:219-225
通讯作者	Hertzler, Philip L.(hertz1pl@cmich.edu)
摘要	Gastrulation occurs by a variety of morphogenetic movements, often correlated with diverse expression of the T-box transcription factor Brachyury (Bra). Bra may be expressed in ectoderm, mesoderm, or endoderm, but its role in cell fate specification or regulation of gastrulation movements has not been studied in the development of crustaceans. Penaeid shrimp (Decapoda: Dendrobranchiata: Penaeidae) develop by complete cleavage and gastrulation by invagination to a free-swimming nauplius larva. Penaeid gastrulation diverges from other decapods and from insects, occurring early at a low cell number with the formation of a radial invagination. Toward a better understanding of gastrulation movements in penaeid shrimp, bra was identified from newly available penaeid shrimp genomes and transcriptomes of Litopenaeus vannamei, Marsupenaeus japonicus, and Penaeus monodon. Additional bra homologs were obtained from the outgroups Sicyonia ingentis (Decapoda: Dendrobranchiata: Sicyoniidae) and the caridean shrimp Caridina multidentata (Decapoda: Pleocymata). The genes encoded penaeid shrimp Bra proteins of 551-552 amino acids, containing the highly conserved T-box DNA-binding region. The N-terminal Smad1-binding domain, conserved in most animals, was absent in shrimp Bra. The R1 repressor domain was the best conserved of the C-terminal regulatory domains, which were widely divergent compared to other species. The penaeid shrimp bra gene consisted of six exons, with splice sites conserved with other phyla across the animal kingdom. Real-time qPCR and FPKM analysis showed that shrimp bra mRNA was strongly expressed during gastrulation. These findings begin to address the evolution of gastrulation in shrimp at the molecular level.
关键词	Shrimp Gastrulation Brachyury T-box
DOI	10.1007/s00427-018-0618-7
收录类别	SCI
语种	英语
资助项目	CSIRO Food Futures Flagship Cluster on Sex ratio and sterility for commercial animal production ; CSIRO Flagship Collaboration Fund ; FRCE grants from Central Michigan University ; National High-Tech Research and Development Program of China (863 Program)[2012AA10A404] ; CSIRO Food Futures Flagship Cluster on Sex ratio and sterility for commercial animal production ; CSIRO Flagship Collaboration Fund ; FRCE grants from Central Michigan University ; National High-Tech Research and Development Program of China (863 Program)[2012AA10A404]
WOS研究方向	Cell Biology ; Evolutionary Biology ; Developmental Biology
WOS类目	Cell Biology ; Evolutionary Biology ; Developmental Biology
WOS记录号	WOS:000442694100004
出版者	SPRINGER
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/159931
专题	实验海洋生物学重点实验室
通讯作者	Hertzler, Philip L.
作者单位	1.Cent Michigan Univ, Dept Biol, Biosci 2100, Mt Pleasant, MI 48858 USA 2.Ocean Univ China, Minist Educ, Key Lab Marine Genet & Breeding, Coll Marine Life Sci, Qingdao 266003, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Hertzler, Philip L.,Wei, Jiankai,Droste, Andrew P.,et al. Penaeid shrimp brachyury: sequence analysis and expression during gastrulation[J]. DEVELOPMENT GENES AND EVOLUTION,2018,228(5):219-225.
APA	Hertzler, Philip L.,Wei, Jiankai,Droste, Andrew P.,Yuan, Jianbo,&Xiang, Jianhai.(2018).Penaeid shrimp brachyury: sequence analysis and expression during gastrulation.DEVELOPMENT GENES AND EVOLUTION,228(5),219-225.
MLA	Hertzler, Philip L.,et al."Penaeid shrimp brachyury: sequence analysis and expression during gastrulation".DEVELOPMENT GENES AND EVOLUTION 228.5(2018):219-225.

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