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Penaeid shrimp brachyury: sequence analysis and expression during gastrulation
Hertzler, Philip L.1; Wei, Jiankai2; Droste, Andrew P.1; Yuan, Jianbo3,4; Xiang, Jianhai3,4
2018-09-01
Source PublicationDEVELOPMENT GENES AND EVOLUTION
ISSN0949-944X
Volume228Issue:5Pages:219-225
Corresponding AuthorHertzler, Philip L.(hertz1pl@cmich.edu)
AbstractGastrulation occurs by a variety of morphogenetic movements, often correlated with diverse expression of the T-box transcription factor Brachyury (Bra). Bra may be expressed in ectoderm, mesoderm, or endoderm, but its role in cell fate specification or regulation of gastrulation movements has not been studied in the development of crustaceans. Penaeid shrimp (Decapoda: Dendrobranchiata: Penaeidae) develop by complete cleavage and gastrulation by invagination to a free-swimming nauplius larva. Penaeid gastrulation diverges from other decapods and from insects, occurring early at a low cell number with the formation of a radial invagination. Toward a better understanding of gastrulation movements in penaeid shrimp, bra was identified from newly available penaeid shrimp genomes and transcriptomes of Litopenaeus vannamei, Marsupenaeus japonicus, and Penaeus monodon. Additional bra homologs were obtained from the outgroups Sicyonia ingentis (Decapoda: Dendrobranchiata: Sicyoniidae) and the caridean shrimp Caridina multidentata (Decapoda: Pleocymata). The genes encoded penaeid shrimp Bra proteins of 551-552 amino acids, containing the highly conserved T-box DNA-binding region. The N-terminal Smad1-binding domain, conserved in most animals, was absent in shrimp Bra. The R1 repressor domain was the best conserved of the C-terminal regulatory domains, which were widely divergent compared to other species. The penaeid shrimp bra gene consisted of six exons, with splice sites conserved with other phyla across the animal kingdom. Real-time qPCR and FPKM analysis showed that shrimp bra mRNA was strongly expressed during gastrulation. These findings begin to address the evolution of gastrulation in shrimp at the molecular level.
KeywordShrimp Gastrulation Brachyury T-box
DOI10.1007/s00427-018-0618-7
Indexed BySCI
Language英语
Funding ProjectCSIRO Food Futures Flagship Cluster on Sex ratio and sterility for commercial animal production ; CSIRO Flagship Collaboration Fund ; FRCE grants from Central Michigan University ; National High-Tech Research and Development Program of China (863 Program)[2012AA10A404]
WOS Research AreaCell Biology ; Evolutionary Biology ; Developmental Biology
WOS SubjectCell Biology ; Evolutionary Biology ; Developmental Biology
WOS IDWOS:000442694100004
PublisherSPRINGER
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159931
Collection实验海洋生物学重点实验室
Corresponding AuthorHertzler, Philip L.
Affiliation1.Cent Michigan Univ, Dept Biol, Biosci 2100, Mt Pleasant, MI 48858 USA
2.Ocean Univ China, Minist Educ, Key Lab Marine Genet & Breeding, Coll Marine Life Sci, Qingdao 266003, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Hertzler, Philip L.,Wei, Jiankai,Droste, Andrew P.,et al. Penaeid shrimp brachyury: sequence analysis and expression during gastrulation[J]. DEVELOPMENT GENES AND EVOLUTION,2018,228(5):219-225.
APA Hertzler, Philip L.,Wei, Jiankai,Droste, Andrew P.,Yuan, Jianbo,&Xiang, Jianhai.(2018).Penaeid shrimp brachyury: sequence analysis and expression during gastrulation.DEVELOPMENT GENES AND EVOLUTION,228(5),219-225.
MLA Hertzler, Philip L.,et al."Penaeid shrimp brachyury: sequence analysis and expression during gastrulation".DEVELOPMENT GENES AND EVOLUTION 228.5(2018):219-225.
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