Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| The pentatricopeptide repeat protein EMPTY PERICARP8 is required for the splicing of three mitochondrial introns and seed development in maize | |
| Sun, Feng1; Zhang, Xiaoyan1; Shen, Yun1; Wang, Hongchun1; Liu, Rui1; Wang, Xiaomin2; Gao, Dahai3; Yang, Yan-Zhuo1; Liu, Yiwei1; Tan, Bao-Cai1 | |
| 2018-09-01 | |
| 发表期刊 | PLANT JOURNAL
 |
| ISSN | 0960-7412 |
| 卷号 | 95期号:5页码:919-932 |
| 通讯作者 | Tan, Bao-Cai(bctan@sdu.edu.cn) |
| 摘要 | Splicing of plant organellar group II introns is under accurate nuclear control that employs many nucleus-encoded protein cofactors from various families. For mitochondrial introns, only a few splicing factors have been characterized because disruption of their functions often causes embryo lethality. Here, we report the function of Empty Pericarp8 (Emp8) in the splicing of three group II introns in mitochondria, complex I biogenesis, and seed development in maize. Emp8 encodes a P subfamily pentatricopeptide repeat protein that localizes in mitochondria. The loss-of-function mutants of Emp8 are embryo lethal, showing severely arrested embryo and endosperm development in maize. The respiration rate in the emp8 mutants is reduced with substantially enhanced expression of alternative oxidases. Transcript analysis indicated that the trans-splicing of nad1 intron 4 and cis-splicing of nad4 intron 1 are abolished, and the cis-splicing of nad2 intron 1 is severely impaired in the emp8 mutants. These defects consequently lead to the disassembly of mitochondrial complex I and a dramatic reduction in its activity. Together, these results suggest that Emp8 is required for the trans-splicing of nad1 intron 4 and cis-splicing of nad4 intron 1 and nad2 intron 1, which is essential to mitochondrial complex I assembly and hence to embryogenesis and endosperm development in maize. |
| 关键词 | maize empty pericarp8 PPR mitochondrion complex I seed development |
| DOI | 10.1111/tpj.14030 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 资助项目 | National Key Research and Development Program of China[2016YFD0101003] ; National Natural Science Foundation of China[91735301] ; National Natural Science Foundation of China[31630053] ; National Natural Science Foundation of China[31500199] ; Shandong postdoctoral innovation fund[201501012] ; Fundamental Research Funds of Shandong University[2015HW010] ; National Key Research and Development Program of China[2016YFD0101003] ; National Natural Science Foundation of China[91735301] ; National Natural Science Foundation of China[31630053] ; National Natural Science Foundation of China[31500199] ; Shandong postdoctoral innovation fund[201501012] ; Fundamental Research Funds of Shandong University[2015HW010] |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| WOS记录号 | WOS:000441759400014 |
| 出版者 | WILEY |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qdio.ac.cn/handle/337002/159890 |
| 专题 | 实验海洋生物学重点实验室 |
| 通讯作者 | Tan, Bao-Cai |
| 作者单位 | 1.Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Peoples R China 2.Lanzhou Univ, Sch Life Sci, Key Lab Cell Act & Stress Adaptat, Minist Educ, Lanzhou 730000, Gansu, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Feng,Zhang, Xiaoyan,Shen, Yun,et al. The pentatricopeptide repeat protein EMPTY PERICARP8 is required for the splicing of three mitochondrial introns and seed development in maize[J]. PLANT JOURNAL,2018,95(5):919-932. |
| APA | Sun, Feng.,Zhang, Xiaoyan.,Shen, Yun.,Wang, Hongchun.,Liu, Rui.,...&Tan, Bao-Cai.(2018).The pentatricopeptide repeat protein EMPTY PERICARP8 is required for the splicing of three mitochondrial introns and seed development in maize.PLANT JOURNAL,95(5),919-932. |
| MLA | Sun, Feng,et al."The pentatricopeptide repeat protein EMPTY PERICARP8 is required for the splicing of three mitochondrial introns and seed development in maize".PLANT JOURNAL 95.5(2018):919-932. |
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| The pentatricopeptid（2329KB） | 期刊论文 | 出版稿 | 限制开放 | CC BY-NC-SA | 浏览 | |
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