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The pentatricopeptide repeat protein EMPTY PERICARP8 is required for the splicing of three mitochondrial introns and seed development in maize
Sun, Feng1; Zhang, Xiaoyan1; Shen, Yun1; Wang, Hongchun1; Liu, Rui1; Wang, Xiaomin2; Gao, Dahai3; Yang, Yan-Zhuo1; Liu, Yiwei1; Tan, Bao-Cai1
2018-09-01
Source PublicationPLANT JOURNAL
ISSN0960-7412
Volume95Issue:5Pages:919-932
Corresponding AuthorTan, Bao-Cai(bctan@sdu.edu.cn)
AbstractSplicing of plant organellar group II introns is under accurate nuclear control that employs many nucleus-encoded protein cofactors from various families. For mitochondrial introns, only a few splicing factors have been characterized because disruption of their functions often causes embryo lethality. Here, we report the function of Empty Pericarp8 (Emp8) in the splicing of three group II introns in mitochondria, complex I biogenesis, and seed development in maize. Emp8 encodes a P subfamily pentatricopeptide repeat protein that localizes in mitochondria. The loss-of-function mutants of Emp8 are embryo lethal, showing severely arrested embryo and endosperm development in maize. The respiration rate in the emp8 mutants is reduced with substantially enhanced expression of alternative oxidases. Transcript analysis indicated that the trans-splicing of nad1 intron 4 and cis-splicing of nad4 intron 1 are abolished, and the cis-splicing of nad2 intron 1 is severely impaired in the emp8 mutants. These defects consequently lead to the disassembly of mitochondrial complex I and a dramatic reduction in its activity. Together, these results suggest that Emp8 is required for the trans-splicing of nad1 intron 4 and cis-splicing of nad4 intron 1 and nad2 intron 1, which is essential to mitochondrial complex I assembly and hence to embryogenesis and endosperm development in maize.
Keywordmaize empty pericarp8 PPR mitochondrion complex I seed development
DOI10.1111/tpj.14030
Indexed BySCI
Language英语
Funding ProjectNational Key Research and Development Program of China[2016YFD0101003] ; National Natural Science Foundation of China[91735301] ; National Natural Science Foundation of China[31630053] ; National Natural Science Foundation of China[31500199] ; Shandong postdoctoral innovation fund[201501012] ; Fundamental Research Funds of Shandong University[2015HW010]
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000441759400014
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159890
Collection实验海洋生物学重点实验室
Corresponding AuthorTan, Bao-Cai
Affiliation1.Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Peoples R China
2.Lanzhou Univ, Sch Life Sci, Key Lab Cell Act & Stress Adaptat, Minist Educ, Lanzhou 730000, Gansu, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
Recommended Citation
GB/T 7714
Sun, Feng,Zhang, Xiaoyan,Shen, Yun,et al. The pentatricopeptide repeat protein EMPTY PERICARP8 is required for the splicing of three mitochondrial introns and seed development in maize[J]. PLANT JOURNAL,2018,95(5):919-932.
APA Sun, Feng.,Zhang, Xiaoyan.,Shen, Yun.,Wang, Hongchun.,Liu, Rui.,...&Tan, Bao-Cai.(2018).The pentatricopeptide repeat protein EMPTY PERICARP8 is required for the splicing of three mitochondrial introns and seed development in maize.PLANT JOURNAL,95(5),919-932.
MLA Sun, Feng,et al."The pentatricopeptide repeat protein EMPTY PERICARP8 is required for the splicing of three mitochondrial introns and seed development in maize".PLANT JOURNAL 95.5(2018):919-932.
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