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An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development
Tan, Sujian1,3; Huan, Pin1; Liu, Baozhong1,2
2018-07-15
Source PublicationGENE
ISSN0378-1119
Volume663Pages:65-71
Corresponding AuthorHuan, Pin(huanpin@qdio.ac.cn)
AbstractThough the roles of BMP signaling in development is studied extensively in insects and vertebrates, our knowledge of BMP signaling in molluscan development is limited. In the present study, we performed a genome-based analysis of TGF-beta receptors in the Pacific oyster Crassostrea gigas and revealed that C. gigas possessed all five canonical members of the gene family, including three type I and two type II receptors. Whole mount in situ hybridization revealed that four receptor genes exhibited universal expression at the gastrula stage but cgi-bmprll mRNA was only expressed at the anterior lip of blastopore, indicating the regulation of BMP signaling through restricted expression of TGF-beta receptors. Treatment of oyster embryos using a BMP signaling inhibitor (dorsomorphin) resulted in obvious changes of the expression of developmental regulatory genes. In particular, three dorsally expressed genes were inhibited and two genes expressed at the opposite side showed increased expression, indicating BMP signaling may function in dorsal-ventral (DV) patterning. Some of the influenced genes were potential shell-formation (pSF) genes, including the well-accepted pSF gene engrailed, and thus this indicates the development of shell field was affected by dorsomorphin treatment. Subsequent scanning electronic observation revealed morphological change of the posterior margin of the shell field. Taken together, these results indicate the roles of BMP signaling in both DV patterning and development of shell field in oyster embryo, which expands our knowledge of early molluscan development.
KeywordBMP signaling Dorsomorphin Dorsal-ventral patterning Shell field Mollusc
DOI10.1016/j.gene.2018.04.035
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China (NSFC)[31472265] ; National Natural Science Foundation of China (NSFC)[41776157]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000434901700008
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159372
Collection实验海洋生物学重点实验室
Corresponding AuthorHuan, Pin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Tan, Sujian,Huan, Pin,Liu, Baozhong. An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development[J]. GENE,2018,663:65-71.
APA Tan, Sujian,Huan, Pin,&Liu, Baozhong.(2018).An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development.GENE,663,65-71.
MLA Tan, Sujian,et al."An investigation of oyster TGF-beta receptor genes and their potential roles in early molluscan development".GENE 663(2018):65-71.
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