First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent

doi:10.1016/j.dci.2018.04.019

IOCAS-IR > 实验海洋生物学重点实验室

	First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent
	Jin, Qian-wen 1,2,3; Sun, Qing-lei1,2 ; Zhang, Jian 1,2; Sun, Li 1,2
	2018-09-01
发表期刊	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN	0145-305X
卷号	86 页码:17-25
通讯作者	Sun, Qing-lei(lsun@qdio.ac.cn)
摘要	C-type lectins (CTLs) play an important role in innate immune defense. In this study, we identified and characterized two CTLs (Lec1 and Lec2) from the tubeworm Alaysia sp. collected from a hydrothermal vent in Pacmanus. Lec1 and Lec2 possess the typical CTL domain but share low sequence identities (10.8% -20.4%) with known CTLs. Recombinant (r) of Lec1 and Lec2 bound to various PAMPs and a wide arrange of bacteria from neritic and deep-sea environments in a Ca2+-independent manner, but only rLec1 caused agglutination of the bound bacteria. The activities of rLec1 and rLec2 were most stable and highest at 4 degrees C, the ambient temperature of the hydrothermal vent, and decreased at higher temperatures. Both lectins inhibited bacterial growth in a highly selective manner and agglutinated the erythrocytes of fish, rabbit, and chicken in a Ca2+-dependent manner. These results provided the first insights into the functional properties of CTLs in deep-sea Alaysia sp. (C) 2018 Elsevier Ltd. All rights reserved.
关键词	C-type lectin Alaysia sp. Deep-sea hydrothermal vent Antibacterial Hemagglutination
DOI	10.1016/j.dci.2018.04.019
收录类别	SCI
语种	英语
资助项目	Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA11030401] ; Taishan Scholar Program of Shandong Province ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA11030401] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Fisheries ; Immunology ; Zoology
WOS类目	Fisheries ; Immunology ; Zoology
WOS记录号	WOS:000435060900003
出版者	ELSEVIER SCI LTD
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/159363
专题	实验海洋生物学重点实验室
通讯作者	Sun, Qing-lei
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Jin, Qian-wen,Sun, Qing-lei,Zhang, Jian,et al. First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,86:17-25.
APA	Jin, Qian-wen,Sun, Qing-lei,Zhang, Jian,&Sun, Li.(2018).First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,86,17-25.
MLA	Jin, Qian-wen,et al."First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 86(2018):17-25.

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