Institutional Repository of Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
| First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent | |
Jin, Qian-wen1,2,3; Sun, Qing-lei1,2 ; Zhang, Jian1,2; Sun, Li1,2
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| 2018-09-01 | |
| Source Publication | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| ISSN | 0145-305X |
| Volume | 86Pages:17-25 |
| Corresponding Author | Sun, Qing-lei(lsun@qdio.ac.cn) |
| Abstract | C-type lectins (CTLs) play an important role in innate immune defense. In this study, we identified and characterized two CTLs (Lec1 and Lec2) from the tubeworm Alaysia sp. collected from a hydrothermal vent in Pacmanus. Lec1 and Lec2 possess the typical CTL domain but share low sequence identities (10.8% -20.4%) with known CTLs. Recombinant (r) of Lec1 and Lec2 bound to various PAMPs and a wide arrange of bacteria from neritic and deep-sea environments in a Ca2+-independent manner, but only rLec1 caused agglutination of the bound bacteria. The activities of rLec1 and rLec2 were most stable and highest at 4 degrees C, the ambient temperature of the hydrothermal vent, and decreased at higher temperatures. Both lectins inhibited bacterial growth in a highly selective manner and agglutinated the erythrocytes of fish, rabbit, and chicken in a Ca2+-dependent manner. These results provided the first insights into the functional properties of CTLs in deep-sea Alaysia sp. (C) 2018 Elsevier Ltd. All rights reserved. |
| Keyword | C-type lectin Alaysia sp. Deep-sea hydrothermal vent Antibacterial Hemagglutination |
| DOI | 10.1016/j.dci.2018.04.019 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA11030401] ; Taishan Scholar Program of Shandong Province ; Qingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA11030401] ; Taishan Scholar Program of Shandong Province |
| WOS Research Area | Fisheries ; Immunology ; Zoology |
| WOS Subject | Fisheries ; Immunology ; Zoology |
| WOS ID | WOS:000435060900003 |
| Publisher | ELSEVIER SCI LTD |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qdio.ac.cn/handle/337002/159363 |
| Collection | 实验海洋生物学重点实验室 |
| Corresponding Author | Sun, Qing-lei |
| Affiliation | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China |
| First Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Corresponding Author Affilication | Institute of Oceanology, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Jin, Qian-wen,Sun, Qing-lei,Zhang, Jian,et al. First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,86:17-25. |
| APA | Jin, Qian-wen,Sun, Qing-lei,Zhang, Jian,&Sun, Li.(2018).First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,86,17-25. |
| MLA | Jin, Qian-wen,et al."First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 86(2018):17-25. |
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| First characterizati（4183KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View | |
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