IOCAS-IR  > 实验海洋生物学重点实验室
First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent
Jin, Qian-wen1,2,3; Sun, Qing-lei1,2; Zhang, Jian1,2; Sun, Li1,2
2018-09-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume86Pages:17-25
Corresponding AuthorSun, Qing-lei(lsun@qdio.ac.cn)
AbstractC-type lectins (CTLs) play an important role in innate immune defense. In this study, we identified and characterized two CTLs (Lec1 and Lec2) from the tubeworm Alaysia sp. collected from a hydrothermal vent in Pacmanus. Lec1 and Lec2 possess the typical CTL domain but share low sequence identities (10.8% -20.4%) with known CTLs. Recombinant (r) of Lec1 and Lec2 bound to various PAMPs and a wide arrange of bacteria from neritic and deep-sea environments in a Ca2+-independent manner, but only rLec1 caused agglutination of the bound bacteria. The activities of rLec1 and rLec2 were most stable and highest at 4 degrees C, the ambient temperature of the hydrothermal vent, and decreased at higher temperatures. Both lectins inhibited bacterial growth in a highly selective manner and agglutinated the erythrocytes of fish, rabbit, and chicken in a Ca2+-dependent manner. These results provided the first insights into the functional properties of CTLs in deep-sea Alaysia sp. (C) 2018 Elsevier Ltd. All rights reserved.
KeywordC-type lectin Alaysia sp. Deep-sea hydrothermal vent Antibacterial Hemagglutination
DOI10.1016/j.dci.2018.04.019
Indexed BySCI
Language英语
Funding ProjectQingdao National Laboratory for Marine Science and Technology[QNLM2016ORP0309] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA11030401] ; Taishan Scholar Program of Shandong Province
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000435060900003
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159363
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Qing-lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Jin, Qian-wen,Sun, Qing-lei,Zhang, Jian,et al. First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,86:17-25.
APA Jin, Qian-wen,Sun, Qing-lei,Zhang, Jian,&Sun, Li.(2018).First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,86,17-25.
MLA Jin, Qian-wen,et al."First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 86(2018):17-25.
Files in This Item:
File Name/Size DocType Version Access License
First characterizati(4183KB)期刊论文出版稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jin, Qian-wen]'s Articles
[Sun, Qing-lei]'s Articles
[Zhang, Jian]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jin, Qian-wen]'s Articles
[Sun, Qing-lei]'s Articles
[Zhang, Jian]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jin, Qian-wen]'s Articles
[Sun, Qing-lei]'s Articles
[Zhang, Jian]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: First characterization of two C-type lectins of the tubeworm Alaysia sp from a deep-sea hydrothermal vent.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.