Population resequencing reveals candidate genes associated with salinity adaptation of the Pacific oyster Crassostrea gigas

doi:10.1038/s41598-018-26953-w

IOCAS-IR

	Population resequencing reveals candidate genes associated with salinity adaptation of the Pacific oyster Crassostrea gigas
	She, Zhicai 3; Li, Li 1,2,5; Meng, Jie 1,2,5; Jia, Zhen 3; Que, Huayong 1,5; Zhang, Guofan 1,4,5
	2018-06-06
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	8 页码:10
通讯作者	Li, Li(lili@qdio.ac.cn) ; Zhang, Guofan(gfzhang@qdio.ac.cn)
摘要	The Pacific oyster Crassostrea gigas is an important cultivated shellfish. As a euryhaline species, it has evolved adaptive mechanisms responding to the complex and changeable intertidal environment that it inhabits. To investigate the genetic basis of this salinity adaptation mechanism, we conducted a genome-wide association study using phenotypically differentiated populations (hyposalinity and hypersalinity adaptation populations, and control population), and confirmed our results using an independent population, high-resolution melting, and mRNA expression analysis. For the hyposalinity adaptation, we determined 24 genes, including Cg_CLCN7 (chloride channel protein 7) and Cg_AP1 (apoptosis 1 inhibitor), involved in the ion/water channel and transporter mechanisms, free amino acid and reactive oxygen species metabolism, immune responses, and chemical defence. Three SNPs located on these two genes were significantly differentiated between groups, as was Cg_CLCN7. For the hypersalinity adaptation, the biological process for positive regulating the developmental process was enriched. Enriched gene functions were focused on transcriptional regulation, signal transduction, and cell growth and differentiation, including calmodulin (Cg_CaM) and ficolin-2 (Cg_FCN2). These genes and polymorphisms possibly play an important role in oyster hyposalinity and hypersalinity adaptation. They not only further our understanding of salinity adaptation mechanisms but also provide markers for highly adaptable oyster strains suitable for breeding.
DOI	10.1038/s41598-018-26953-w
收录类别	SCI
语种	英语
资助项目	Key Laboratory of Beibu Gulf Marine Biodiversity Conservation Program of Guangxi[2018ZB07] ; Education Department Program of Guangxi[KY2016YB490] ; earmarked fund for Modern Agro-industry Technology Research System[CARS-49] ; National Basic Research Program of China (973 Program)[2010CB126402] ; National Natural Science Foundation of China[31530079] ; National Natural Science Foundation of China[31572620] ; National Natural Science Foundation of China[31572620] ; National Natural Science Foundation of China[31530079] ; National Basic Research Program of China (973 Program)[2010CB126402] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] ; Education Department Program of Guangxi[KY2016YB490] ; Key Laboratory of Beibu Gulf Marine Biodiversity Conservation Program of Guangxi[2018ZB07]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000434252000019
出版者	NATURE PUBLISHING GROUP
WOS关键词	GENOME-WIDE ASSOCIATION ; LOCAL ADAPTATION ; ATLANTIC SALMON ; EXPRESSION ; STRESS ; IDENTIFICATION ; TRANSPORTER ; RESPONSES ; SELECTION ; ALIGNMENT
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/159235
通讯作者	Li, Li; Zhang, Guofan
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao 266071, Shandong, Peoples R China 3.Qinzhou Univ, Guangxi Key Lab Beibu Gulf Marine Biodivers Conse, Qinzhou 535011, Guangxi, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Shandong, Peoples R China 5.Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	She, Zhicai,Li, Li,Meng, Jie,et al. Population resequencing reveals candidate genes associated with salinity adaptation of the Pacific oyster Crassostrea gigas[J]. SCIENTIFIC REPORTS,2018,8:10.
APA	She, Zhicai,Li, Li,Meng, Jie,Jia, Zhen,Que, Huayong,&Zhang, Guofan.(2018).Population resequencing reveals candidate genes associated with salinity adaptation of the Pacific oyster Crassostrea gigas.SCIENTIFIC REPORTS,8,10.
MLA	She, Zhicai,et al."Population resequencing reveals candidate genes associated with salinity adaptation of the Pacific oyster Crassostrea gigas".SCIENTIFIC REPORTS 8(2018):10.