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A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp
Guo, Qing1,2,3; Li, Shihao1,2; Lv, Xinjia1,3; Xiang, Jianhai1,2; Sagi, Amir4; Manor, Rivka4; Li, Fuhua1,2
2018-05-01
Source PublicationENDOCRINOLOGY
ISSN0013-7227
Volume159Issue:5Pages:2173-2185
Corresponding AuthorLi, Shihao(lishihao@qdio.ac.cn) ; Li, Fuhua(fhli@qdio.ac.cn)
AbstractThe insulin-like androgenic gland hormone (IAG) is the key regulator in crustacean male sexual differentiation. As a secreted peptide hormone, IAG might perform its biological function through interacting with the membrane receptor. However, the receptor of IAG remains unclear. In the current study, a putative IAG receptor gene (FcIAGR) was identified in Fenneropenaeus chinensis. The deduced amino acid sequence of FcIAGR contained several conserved domains of insulin-like receptor proteins, including two L domains (L1 and L2), a cysteine-rich domain, three fibronectin III domains, a transmembrane domain, and an intracellular tyrosine kinase domain. Tissue distribution and in situ hybridization analysis showed that FcIAGR was predominantly expressed in the androgenic gland and testis in male F. chinensis. Protein colocalization analysis in HEK293 cells showed that FcIAGR could colocalize with both FcIAG1 and FcIAG2, respectively. Yeast two-hybrid assay further confirmed the interactions between FcIAGR and FcIAGs. After a long-term silencing of FcIAGR with double-stranded RNA, most of the germ cells in the testis were arrested at the secondary spermatocytes, whereas those in the control developed into sperm cells. The data indicated that FcIAGR was the receptor of FcIAGs in F. chinensis. The current study provides insight into the mechanism that the insulin-like signaling pathway regulates the male sexual differentiation in Decapoda crustaceans.
DOI10.1210/en.2017-03253
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31461143007] ; National Natural Science Foundation of China[31302171] ; Blue Life Breakthrough Program of LMBB of Qingdao National Laboratory for Marine Science and Technology[MS2017NO04]
WOS Research AreaEndocrinology & Metabolism
WOS SubjectEndocrinology & Metabolism
WOS IDWOS:000432685100024
PublisherOXFORD UNIV PRESS INC
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159184
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Shihao; Li, Fuhua
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Guo, Qing,Li, Shihao,Lv, Xinjia,et al. A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp[J]. ENDOCRINOLOGY,2018,159(5):2173-2185.
APA Guo, Qing.,Li, Shihao.,Lv, Xinjia.,Xiang, Jianhai.,Sagi, Amir.,...&Li, Fuhua.(2018).A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp.ENDOCRINOLOGY,159(5),2173-2185.
MLA Guo, Qing,et al."A Putative Insulin-like Androgenic Gland Hormone Receptor Gene Specifically Expressed in Male Chinese Shrimp".ENDOCRINOLOGY 159.5(2018):2173-2185.
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