Wnt gene family members and their expression profiling in Litopenaeus vannamei

doi:10.1016/j.fsi.2018.03.034

IOCAS-IR > 实验海洋生物学重点实验室

	Wnt gene family members and their expression profiling in Litopenaeus vannamei
	Du, Jiangli1,2,3 ; Zhang, Xiaojun 1,2; Yuan, Jianbo 1,2; Zhang, Xiaoxi 1,2,3; Li, Fuhua 1,2; Xiang, Jianhai1,2
	2018-06-01
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
卷号	77 页码:233-243
通讯作者	Zhang, Xiaojun(xjzhang@qdio.ac.cn)
摘要	The Wnt gene family encodes secreted glycoproteins involved in a wide variety of biological processes, including embryo development, cell proliferation and differentiation, and tissue regeneration. The Wnt pathway exists in all metazoan animals, however, the relevant research is rare in crustaceans. Here we described 12 Wnt genes representing 12 Wnt gene subfamilies in the Pacific white shrimp, Litopenaeus vannamei. Based on homolog annotations and phylogenetic analyses, we named these 12 Wnt genes as LvWnt1, LvWnt2, LvWnt4-11, LvWnt16, and LvWntA. All the corresponding LvWnt proteins shared a conserved Wnt1 domain and 22 conserved cysteine residues. LvWnt1 and LvWnt6 were adjacent in a scaffold in the shrimp genome. Furthermore, we performed expression analyses of LvWnt genes at different developmental stages, during the molting process, in different tissues and after different pathogenic infection. We showed that each LvWnt gene had a unique expression pattern at different developmental stages but only a few of them expressed in adult shrimp. All the investigated LvWnt genes were initially expressed at the gastrula or limb bud embryo stages. Among them, LvWnt5 was specifically high expressed only in early embryos. LvWntA and LvWnt5 displayed high and similar expression profiles during the molting process, and LvWnt6 and LvWnt16 were specifically expressed in the thoracic ganglion, ventral nerve, intestines and gill tissues, respectively. We also found the expression of LvWntA, LvWnt5, LvWnt6, LvWnt9, and LvWnt16 were varied in the different tissues after infected with Staphylococcus aureus, Vibrio parahaemolyticus and white spot syndrome virus (WSSV), which indicated that they might participate in immune response in L. vannamei. This study provided an insight into the repertoire of the Wnt gene structure and expression in shrimps, and furthermore, might promote the understanding of development, growth and immune response of shrimps and crustaceans.
关键词	Litopenaeus vannamei Wnt gene family Expression pattern Development Immunity
DOI	10.1016/j.fsi.2018.03.034
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31672632] ; National Natural Science Foundation of China[41506189] ; National Natural Science Foundation of China[41376165] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03] ; Qingdao National Laboratory for Marine Science and Technology[CARS-47] ; National Natural Science Foundation of China[31672632] ; National Natural Science Foundation of China[41506189] ; National Natural Science Foundation of China[41376165] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03] ; Qingdao National Laboratory for Marine Science and Technology[CARS-47]
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS类目	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000432644300027
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/159161
专题	实验海洋生物学重点实验室
通讯作者	Zhang, Xiaojun
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Qingdao 266237, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位	实验海洋生物学重点实验室
通讯作者单位	实验海洋生物学重点实验室
推荐引用方式 GB/T 7714	Du, Jiangli,Zhang, Xiaojun,Yuan, Jianbo,et al. Wnt gene family members and their expression profiling in Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2018,77:233-243.
APA	Du, Jiangli,Zhang, Xiaojun,Yuan, Jianbo,Zhang, Xiaoxi,Li, Fuhua,&Xiang, Jianhai.(2018).Wnt gene family members and their expression profiling in Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,77,233-243.
MLA	Du, Jiangli,et al."Wnt gene family members and their expression profiling in Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 77(2018):233-243.

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