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Wnt gene family members and their expression profiling in Litopenaeus vannamei
Du, Jiangli1,2,3; Zhang, Xiaojun1,2; Yuan, Jianbo1,2; Zhang, Xiaoxi1,2,3; Li, Fuhua1,2; Xiang, Jianhai1,2
2018-06-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume77Pages:233-243
Corresponding AuthorZhang, Xiaojun(xjzhang@qdio.ac.cn)
AbstractThe Wnt gene family encodes secreted glycoproteins involved in a wide variety of biological processes, including embryo development, cell proliferation and differentiation, and tissue regeneration. The Wnt pathway exists in all metazoan animals, however, the relevant research is rare in crustaceans. Here we described 12 Wnt genes representing 12 Wnt gene subfamilies in the Pacific white shrimp, Litopenaeus vannamei. Based on homolog annotations and phylogenetic analyses, we named these 12 Wnt genes as LvWnt1, LvWnt2, LvWnt4-11, LvWnt16, and LvWntA. All the corresponding LvWnt proteins shared a conserved Wnt1 domain and 22 conserved cysteine residues. LvWnt1 and LvWnt6 were adjacent in a scaffold in the shrimp genome. Furthermore, we performed expression analyses of LvWnt genes at different developmental stages, during the molting process, in different tissues and after different pathogenic infection. We showed that each LvWnt gene had a unique expression pattern at different developmental stages but only a few of them expressed in adult shrimp. All the investigated LvWnt genes were initially expressed at the gastrula or limb bud embryo stages. Among them, LvWnt5 was specifically high expressed only in early embryos. LvWntA and LvWnt5 displayed high and similar expression profiles during the molting process, and LvWnt6 and LvWnt16 were specifically expressed in the thoracic ganglion, ventral nerve, intestines and gill tissues, respectively. We also found the expression of LvWntA, LvWnt5, LvWnt6, LvWnt9, and LvWnt16 were varied in the different tissues after infected with Staphylococcus aureus, Vibrio parahaemolyticus and white spot syndrome virus (WSSV), which indicated that they might participate in immune response in L. vannamei. This study provided an insight into the repertoire of the Wnt gene structure and expression in shrimps, and furthermore, might promote the understanding of development, growth and immune response of shrimps and crustaceans.
KeywordLitopenaeus vannamei Wnt gene family Expression pattern Development Immunity
DOI10.1016/j.fsi.2018.03.034
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31672632] ; National Natural Science Foundation of China[41506189] ; National Natural Science Foundation of China[41376165] ; Qingdao National Laboratory for Marine Science and Technology[2015ASKJ02-03] ; Qingdao National Laboratory for Marine Science and Technology[CARS-47]
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000432644300027
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/159161
Collection实验海洋生物学重点实验室
Corresponding AuthorZhang, Xiaojun
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Qingdao 266237, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Du, Jiangli,Zhang, Xiaojun,Yuan, Jianbo,et al. Wnt gene family members and their expression profiling in Litopenaeus vannamei[J]. FISH & SHELLFISH IMMUNOLOGY,2018,77:233-243.
APA Du, Jiangli,Zhang, Xiaojun,Yuan, Jianbo,Zhang, Xiaoxi,Li, Fuhua,&Xiang, Jianhai.(2018).Wnt gene family members and their expression profiling in Litopenaeus vannamei.FISH & SHELLFISH IMMUNOLOGY,77,233-243.
MLA Du, Jiangli,et al."Wnt gene family members and their expression profiling in Litopenaeus vannamei".FISH & SHELLFISH IMMUNOLOGY 77(2018):233-243.
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