First comprehensive analysis of lysine acetylation in Alvinocaris longirostris from the deep-sea hydrothermal vents

doi:10.1186/s12864-018-4745-3

	First comprehensive analysis of lysine acetylation in Alvinocaris longirostris from the deep-sea hydrothermal vents
	Hui, Min 1,2; Cheng, Jiao 1,2; Sha, Zhongli1,2,3
	2018-05-10
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	19 页码:13
摘要	Background: Deep-sea hydrothermal vents are unique chemoautotrophic ecosystems with harsh conditions. Alvinocaris longirostris is one of the dominant crustacean species inhabiting in these extreme environments. It is significant to clarify mechanisms in their adaptation to the vents. Lysine acetylation has been known to play critical roles in the regulation of many cellular processes. However, its function in A. longirostris and even marine invertebrates remains elusive. Our study is the first, to our knowledge, to comprehensively investigate lysine acetylome in A. longirostris. Results: In total, 501 unique acetylation sites from 206 proteins were identified by combination of affinity enrichment and high-sensitive-massspectrometer. It was revealed that Arg, His and Lys occurred most frequently at the + 1 position downstream of the acetylation sites, which were all alkaline amino acids and positively charged. Functional analysis revealed that the protein acetylation was involved in diverse cellular processes, such as biosynthesis of amino acids, citrate cycle, fatty acid degradation and oxidative phosphorylation. Acetylated proteins were found enriched in mitochondrion and peroxisome, and many stress response related proteins were also discovered to be acetylated, like arginine kinases, heat shock protein 70, and hemocyanins. In the two hemocyanins, nine acetylation sites were identified, among which one acetylation site was unique in A. longirostris when compared with other shallow water shrimps. Further studies are warranted to verify its function. Conclusion: The lysine acetylome of A. longirostris is investigated for the first time and brings new insights into the regulation function of the lysine acetylation. The results supply abundant resources for exploring the functions of acetylation in A. longirostris and other shrimps.
关键词	Alvinocarididae Hemocyanins Hydrothermal vent Lysine acetylation Post-translational modification
DOI	10.1186/s12864-018-4745-3
收录类别	SCI
语种	英语
资助项目	Chinese Academy of Sciences[XDA1103040102] ; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC036] ; Key Research Program of Frontier Sciences, Chinese Academy of Sciences[QYZDB-SSW-DQC036] ; Chinese Academy of Sciences[XDA1103040102]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000431831000004
出版者	BIOMED CENTRAL LTD
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
版本	出版稿
条目标识符	http://ir.qdio.ac.cn/handle/337002/158984
专题	海洋生物分类与系统演化实验室深海极端环境与生命过程研究中心
通讯作者	Sha, Zhongli
作者单位	1.Chinese Acad Sci, Inst Oceanol, Lab Marine Organism Taxon & Phylogeny, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Hui, Min,Cheng, Jiao,Sha, Zhongli. First comprehensive analysis of lysine acetylation in Alvinocaris longirostris from the deep-sea hydrothermal vents[J]. BMC GENOMICS,2018,19:13.
APA	Hui, Min,Cheng, Jiao,&Sha, Zhongli.(2018).First comprehensive analysis of lysine acetylation in Alvinocaris longirostris from the deep-sea hydrothermal vents.BMC GENOMICS,19,13.
MLA	Hui, Min,et al."First comprehensive analysis of lysine acetylation in Alvinocaris longirostris from the deep-sea hydrothermal vents".BMC GENOMICS 19(2018):13.

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