Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system

doi:10.1007/s00343-017-6203-5

IOCAS-IR

	Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system
	Wang Chun 1,2; Sun Guoxiang 1; Li Shuangshuang 3; Li Xian 1; Liu Ying 1
	2018-03-01
发表期刊	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN	2096-5508
卷号	36 期号:2 页码:414-426
通讯作者	Liu Ying(yinliu@qdio.ac.cn)
摘要	The present study sampled the intestinal content of healthy and unhealthy Atlantic salmon (Salmo salar L.), the ambient water of unhealthy fish, and the biofilter material in the recirculating aquaculture system (RAS) to understand differences in the intestinal microbiota. The V4-V5 regions of the prokaryotic 16S rRNA genes in the samples were analyzed by MiSeq high-throughput sequencing. The fish were adults with no differences in body length or weight. Representative members of the intestinal microbiota were identified. The intestinal microbiota of the healthy fish included Proteobacteria (44.33%), Actinobacteria (17.89%), Bacteroidetes (15.25%), and Firmicutes (9.11%), among which the families Micrococcaceae and Oxalobacteraceae and genera Sphingomonas, Streptomyces, Pedobacter, Janthinobacterium, Burkholderia, and Balneimonas were most abundant. Proteobacteria (70.46%), Bacteroidetes (7.59%), and Firmicutes (7.55%) dominated the microbiota of unhealthy fish, and Chloroflexi (2.71%), and Aliivibrio and Vibrio as well as genera in the family Aeromonadaceae were most strongly represented. Overall, the intestinal hindgut microbiota differed between healthy and unhealthy fish. This study offers a useful tool for monitoring the health status of fish and for screening the utility of probiotics by studying the intestinal microbiota.
关键词	intestinal microbiota health status Atlantic salmon (Salmo salar L.) recirculating aquaculture system high-throughput pyrosequencing
DOI	10.1007/s00343-017-6203-5
收录类别	SCI
语种	英语
资助项目	Project for International S&T Cooperation Program of China[2014DFA31030] ; Earmarked Fund for Modern Agroindustry Technology Research System[CARS-48] ; National Key Technology Research and Development Program of China[2014BAD08B09] ; Qingdao Innovation Talents Program[13-CX-16] ; National Natural Science Foundation of China[31402283] ; National Natural Science Foundation of China[41306152] ; National Natural Science Foundation of China[31472312] ; National Natural Science Foundation of China[31472312] ; National Natural Science Foundation of China[41306152] ; National Natural Science Foundation of China[31402283] ; Qingdao Innovation Talents Program[13-CX-16] ; National Key Technology Research and Development Program of China[2014BAD08B09] ; Earmarked Fund for Modern Agroindustry Technology Research System[CARS-48] ; Project for International S&T Cooperation Program of China[2014DFA31030]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
WOS类目	Limnology ; Oceanography
WOS记录号	WOS:000431662200021
出版者	SCIENCE PRESS
WOS关键词	TROUT ONCORHYNCHUS-MYKISS ; EARLY-LIFE STAGES ; GUT MICROBIOTA ; AEROMONAS-SALMONICIDA ; BACTERIAL COMMUNITIES ; GRASS CARP ; CTENOPHARYNGODON-IDELLUS ; MOLECULAR ANALYSIS ; FISH LARVAE ; DIVERSITY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/158918
通讯作者	Liu Ying
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hebei Univ Engn, Coll Energy & Environm Engn, Handan 056038, Peoples R China
推荐引用方式 GB/T 7714	Wang Chun,Sun Guoxiang,Li Shuangshuang,et al. Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(2):414-426.
APA	Wang Chun,Sun Guoxiang,Li Shuangshuang,Li Xian,&Liu Ying.(2018).Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(2),414-426.
MLA	Wang Chun,et al."Intestinal microbiota of healthy and unhealthy Atlantic salmon Salmo salar L. in a recirculating aquaculture system".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.2(2018):414-426.