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Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress
Zheng, Rikuan1,2,3; Wu, Shimei4; Ma, Ning1,2,3; Sun, Chaomin1,2
2018-04-05
Source PublicationFRONTIERS IN MICROBIOLOGY
ISSN1664-302X
Volume9Pages:14
Corresponding AuthorSun, Chaomin(sunchaomin@qdio.ac.cn)
AbstractMercury-mediated toxicity remains one of the greatest barriers against microbial survival, even though bacterial resistance to mercury compounds can occur. However, the genetic and physiological adaptations of bacteria to mercury stress still remains unclear. Here, we show that the marine bacterium Pseudomonas stutzeri 273 is resistant to 50 mu M Hg2+ and removes up to 94% Hg2+ from culture. Using gene homologous recombination and complementation, we show that genes encoding Hg2+-transport proteins MerT, MerP, the mercuric reductase MerA and the regulatory protein MerD are essential for bacterial mercuric resistance when challenged with Hg2+. Further, mercury stress inhibits flagellar development, motility, chemotaxis and biofilm formation of P. stutzeri 273, which are verified by transcriptomic and physiological analyses. Surprisingly, we discover that MerF, a previously reported Hg2+-transporter, determines flagellar development, motility and biofilm formation in P. stutzeri 273 by genetic and physiological analyses. Our results strongly indicate that MerF plays an integral role in P. stutzeri 273 to develop physiological responses to mercury stress. Notably, MerF homologs are also prevalent in different human pathogens. Using this unique target may provide novel strategies to control these pathogenic bacteria, given the role of MerF in flagella and biofilm formation. In summary, our data provide an original report on MerF in bacterial physiological development and suggest that the mer in marine bacteria has evolved through progressive, sequential recruitment of novel functions over time.
Keywordmarine Pseudomonas stutzeri mercury stress motility flagella
DOI10.3389/fmicb.2018.00682
Indexed BySCI
Language英语
Funding ProjectTaishan Young Scholar Foundation of Shandong Province[tsqn20161051] ; Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; Natural Science Outstanding Youth Fund of Shandong Province[JQ201607] ; 100-Talent Project of the Chinese Academy of Sciences
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000429289200001
PublisherFRONTIERS MEDIA SA
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158442
Collection实验海洋生物学重点实验室
Corresponding AuthorSun, Chaomin
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
4.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zheng, Rikuan,Wu, Shimei,Ma, Ning,et al. Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress[J]. FRONTIERS IN MICROBIOLOGY,2018,9:14.
APA Zheng, Rikuan,Wu, Shimei,Ma, Ning,&Sun, Chaomin.(2018).Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress.FRONTIERS IN MICROBIOLOGY,9,14.
MLA Zheng, Rikuan,et al."Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress".FRONTIERS IN MICROBIOLOGY 9(2018):14.
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