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CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas
Sun, Rui1; Qiu, Limei1; Yi, Qilin2,3,4; Wang, Mengqiang1; Yue, Feng1; Wang, Lingling2,3; Song, Linsheng2,3,4
2018-03-01
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
Volume74Pages:386-392
Corresponding AuthorWang, Lingling(wanglingling@dlou.edu.cn)
AbstractToll like receptor (TLR) signaling cascades are under precise regulations to ensure the proper immune responses during various pathogen invasions. The neuregulin receptor degradation protein-1 (Nrdp1) has been demonstrated to be a novel negative regulator of TLR signaling by targeting MyD88 to induce degradation in mammals. In the present study, an Nrdp1 homologue, CgNrdp1, was identified from the genome of Pacific oyster Crassostrea gigas. It contained an open reading frame encoding a polypeptide of 315 amino acids which shared high identities with other homologues from different species. There was a conserved RING domain in CgNrdp1, indicating the functional E3 ubiquitin ligase activity. The bacterially expressed recombinant CgNrdp1 and CgMyD88 showed much stronger affinity compared to control groups in the ELISA assay, showing the interacting ability between CgNrdp1 and CgMyD88. When CgMyD88 or HsMyD88 was co-transfected with CgNrdp1 into HEK293T cells, the luciferase activities of NF-kappa B were significantly decreased compared to those in MyD88 single-transfection groups, indicating the conserved negative regulating function of CgNrdp1 on the MyD88 induced TLR signaling. These results indicated that CgNrdp1 was a negative regulator of TLR signaling in oyster and the Nrdp1-MyD88 axis was functional and highly conserved from mollusks to mammals in the negative regulation of TLR signaling.
KeywordNrdp1 Toll like receptor signaling pathway Immune regulation Evolution of innate immunity Crassostrea gigas
DOI10.1016/j.fsi.2017.12.057
Indexed BySCI
Language英语
Funding ProjectNational Science Foundation of China[U1706204] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000427344200041
PublisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158123
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Lingling
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Sun, Rui,Qiu, Limei,Yi, Qilin,et al. CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2018,74:386-392.
APA Sun, Rui.,Qiu, Limei.,Yi, Qilin.,Wang, Mengqiang.,Yue, Feng.,...&Song, Linsheng.(2018).CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,74,386-392.
MLA Sun, Rui,et al."CgNrdp1, a conserved negative regulating factor of MyD88-dependent Toll like receptor signaling in oyster Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 74(2018):386-392.
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