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The cyclin-dependent kinase 2 (CDK2) mediates hematopoiesis through G1-to-S transition in Chinese mitten crab Eriocheir sinensis
Yang, Bin1,3; Jia, Yunke1,3; Jia, Zhihao1,3; Wang, Weilin4; Song, Xiaorui4; Li, Yannan4; Yi, Qilin4; Wang, Lingling2,4; Song, Linsheng2,4
2018-04-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume81Pages:156-166
Corresponding AuthorSong, Linsheng(lshsong@qdio.ac.cn)
AbstractCyclin-dependent kinases (CDKs), a family of cell cycle-related serine/threonine kinases, participate in various biological processes, and play crucial roles in the innate immunity. In the present study, a CDK2 (designed as EsCDK2) with a serine/threonine protein kinase catalytic domain was identified from Chinese mitten crab (Eriocheir sinensis). The full-length cDNA sequence of EsCDK2 was of 2405 bp with an open reading frame (ORF) of 909 bp. EsCDK2 shared 66%-81% sequence similarities with previously identified CDK2s. It was clustered with the CDK2 from Penaeus monodon in the invertebrate branch of the phylogenetic trees The mRNA transcripts of EsCDK2 were highly expressed in hematopoietic tissue (HPT) and(.) gonad, while lower in hemocytes, heart, gills, and muscle. EsCDK2 protein distributed in both cytoplasm and nucleus of HPT cells. The expression of EsCDK2 mRNA in HPT was significantly up regulated and peaked at 3 h post stimulations with Aeromonas hydrophila (2.31-fold, p < 0.05) and Lipopolysaccharide (LPS) (2.02-fold, p < 0.05). After exsanguination, the total hemocyte counts (THC) decreased significantly to 0.42 x 107/ml (0.39-fold, p < 0.05) at 0.5 h, then returned to a normal level at 6 h, while the mRNA expression of EsCDK2 in HPT cells was up-regulated at the early phase from 0.5 h to 6 h. After injection of EsCDK2-dsRNA, the mRNA expression level of EsCDK2 in HPT and THC both decreased to 0.53-fold (p < 0.01) and 0.78-fold (p < 0.05) at 24 h, respectively, and the percentage of new-born hemocytes in HPT also decreased significantly from 37.7% to 16.3% (0.43-fold, p < 0.01). After knocking down of EsCDK2, THC decreased dramatically at 6 h (0.65-fold, p < 0.01) post exsanguination, while returned normal at 6 h in PBS group. After interference of EsCDK2 mRNA expression, the percentage of GO-G1 phase cells significantly increased to 85.01% (1.26-fold, p < 0.01), while S phase and G2 M phase cells significantly decreased to 7.92% (0.46-fold, p < 0.01) and 7.07% (0.43-fold, p < 0.01) respectively, indicating that the cell cycle of HPT cells arrested at G1 phase. These results collectively demonstrated that EsCDK2 participated in the regeneration of hemocytes or hematopoiesis by regulating the transition from G1 to S phase in the cell cycle, and involves in the innate immune responses of E. sinensis. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordEriocheir sinensis Innate immunity CDK2 Cell cycle Hematopoiesis
DOI10.1016/j.dci.2017.11.022
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[31530069] ; Qingdao National Laboratory for Marine Science and Technology[2017ASTCP-OS13] ; Dalian High Level Talent Innovation Support Program[2015R020] ; Research Foundation for Talented Scholars in Dalian Ocean University ; Distinguished Professor of Liaoning
WOS Research AreaFisheries ; Immunology ; Zoology
WOS SubjectFisheries ; Immunology ; Zoology
WOS IDWOS:000424726900016
PublisherELSEVIER SCI LTD
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/158054
Collection实验海洋生物学重点实验室
Corresponding AuthorSong, Linsheng
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China
First Author AffilicationKey Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Yang, Bin,Jia, Yunke,Jia, Zhihao,et al. The cyclin-dependent kinase 2 (CDK2) mediates hematopoiesis through G1-to-S transition in Chinese mitten crab Eriocheir sinensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,81:156-166.
APA Yang, Bin.,Jia, Yunke.,Jia, Zhihao.,Wang, Weilin.,Song, Xiaorui.,...&Song, Linsheng.(2018).The cyclin-dependent kinase 2 (CDK2) mediates hematopoiesis through G1-to-S transition in Chinese mitten crab Eriocheir sinensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,81,156-166.
MLA Yang, Bin,et al."The cyclin-dependent kinase 2 (CDK2) mediates hematopoiesis through G1-to-S transition in Chinese mitten crab Eriocheir sinensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 81(2018):156-166.
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