Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens

doi:10.1111/tpj.13764

IOCAS-IR > 实验海洋生物学重点实验室

	Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens
	Zheng, Zhenbing 1,2,3; Gao, Shan 1,2; Huan, Li1,2 ; Wang, Guang-Ce 1,2
	2018
发表期刊	PLANT JOURNAL
ISSN	0960-7412
卷号	93 期号:1 页码:119-130
通讯作者	Wang, Guang-Ce(gcwang@qdio.ac.cn)
摘要	Due to its highly efficient homologous recombination ability and unusual evolutionary position, the moss Physcomitrella patens has begun to attract more attention in genetic and evolutionary studies. Protonema, the filament stage of the gametophyte, is of great significance in P. patens protoplast isolation. Moreover, protonema is widely used in genetic engineering. However, difficulties in the induction and state maintenance of protonema restrict its wider application. In this work, protonema was induced efficiently in a diluted seawater medium, and the filamentous state was maintained without further cell differentiation. The developmental process of the protonema resumed, progressing to bud assembly and gametophore formation after transfer to freshwater medium. In addition, a transcriptome analysis showed that plant hormone signal transduction pathways were downregulated when protonema was grown in diluted seawater medium. Consistent with the transcriptome results, the protonema failed to respond to the addition of indole-3-acetic acid and 6-benzylaminopurine to the diluted seawater medium. Based on these results, we concluded that diluted seawater medium blocks the differentiation of protonema. This result could provide a novel insight to benefit future protonema production.
关键词	protonema seawater auxin cytokinin Physcomitrella patens
DOI	10.1111/tpj.13764
收录类别	SCI
语种	英语
资助项目	Chinese Academy of Sciences[XDA11020404] ; National Natural Science Foundation of China[41376164] ; National Natural Science Foundation of China[41676157] ; National Natural Science Foundation of China[41606161] ; National Natural Science Foundation of China[41606159] ; National Natural Science Foundation of China[41476140] ; National Natural Science Foundation of China[41506172] ; Chinese Academy of Sciences[XDA11020404] ; National Natural Science Foundation of China[41376164] ; National Natural Science Foundation of China[41676157] ; National Natural Science Foundation of China[41606161] ; National Natural Science Foundation of China[41606159] ; National Natural Science Foundation of China[41476140] ; National Natural Science Foundation of China[41506172]
WOS研究方向	Plant Sciences
WOS类目	Plant Sciences
WOS记录号	WOS:000418228000010
出版者	WILEY
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qdio.ac.cn/handle/337002/157766
专题	实验海洋生物学重点实验室
通讯作者	Wang, Guang-Ce
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
第一作者单位	中国科学院海洋研究所
通讯作者单位	中国科学院海洋研究所
推荐引用方式 GB/T 7714	Zheng, Zhenbing,Gao, Shan,Huan, Li,et al. Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens[J]. PLANT JOURNAL,2018,93(1):119-130.
APA	Zheng, Zhenbing,Gao, Shan,Huan, Li,&Wang, Guang-Ce.(2018).Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens.PLANT JOURNAL,93(1),119-130.
MLA	Zheng, Zhenbing,et al."Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens".PLANT JOURNAL 93.1(2018):119-130.

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