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Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens
Zheng, Zhenbing1,2,3; Gao, Shan1,2; Huan, Li1,2; Wang, Guang-Ce1,2
2018
Source PublicationPLANT JOURNAL
ISSN0960-7412
Volume93Issue:1Pages:119-130
Corresponding AuthorWang, Guang-Ce(gcwang@qdio.ac.cn)
AbstractDue to its highly efficient homologous recombination ability and unusual evolutionary position, the moss Physcomitrella patens has begun to attract more attention in genetic and evolutionary studies. Protonema, the filament stage of the gametophyte, is of great significance in P. patens protoplast isolation. Moreover, protonema is widely used in genetic engineering. However, difficulties in the induction and state maintenance of protonema restrict its wider application. In this work, protonema was induced efficiently in a diluted seawater medium, and the filamentous state was maintained without further cell differentiation. The developmental process of the protonema resumed, progressing to bud assembly and gametophore formation after transfer to freshwater medium. In addition, a transcriptome analysis showed that plant hormone signal transduction pathways were downregulated when protonema was grown in diluted seawater medium. Consistent with the transcriptome results, the protonema failed to respond to the addition of indole-3-acetic acid and 6-benzylaminopurine to the diluted seawater medium. Based on these results, we concluded that diluted seawater medium blocks the differentiation of protonema. This result could provide a novel insight to benefit future protonema production.
Keywordprotonema seawater auxin cytokinin Physcomitrella patens
DOI10.1111/tpj.13764
Indexed BySCI
Language英语
Funding ProjectChinese Academy of Sciences[XDA11020404] ; National Natural Science Foundation of China[41376164] ; National Natural Science Foundation of China[41676157] ; National Natural Science Foundation of China[41606161] ; National Natural Science Foundation of China[41606159] ; National Natural Science Foundation of China[41476140] ; National Natural Science Foundation of China[41506172]
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000418228000010
PublisherWILEY
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/157766
Collection实验海洋生物学重点实验室
Corresponding AuthorWang, Guang-Ce
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Zheng, Zhenbing,Gao, Shan,Huan, Li,et al. Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens[J]. PLANT JOURNAL,2018,93(1):119-130.
APA Zheng, Zhenbing,Gao, Shan,Huan, Li,&Wang, Guang-Ce.(2018).Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens.PLANT JOURNAL,93(1),119-130.
MLA Zheng, Zhenbing,et al."Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens".PLANT JOURNAL 93.1(2018):119-130.
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