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Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)
Song, Hao1,2,3; Qi, Lu4; Zhang, Tao1,2; Wang, Hai-yan1,2
2017-12-01
Source PublicationG3-GENES GENOMES GENETICS
ISSN2160-1836
Volume7Issue:12Pages:3999-4008
Corresponding AuthorZhang, Tao(tzhang@qdio.ac.cn) ; Wang, Hai-yan(haiyanwang@qdio.ac.cn)
AbstractThe veined rapa whelk (Rapana venosa) is widely consumed in China. Nevertheless, it preys on oceanic bivalves, thereby reducing this resource worldwide. Its larval metamorphosis comprises a transition from pelagic to benthic form, which involves considerable physiological and structural changes and has vital roles in its natural populations and commercial breeding. Thus, understanding the endogenous microRNAs (miRNAs) that drive metamorphosis is of great interest. This is the first study to use high-throughput sequencing to examine the alterations in miRNA expression that occur during metamorphosis in a marine gastropod. A total of 195 differentially expressed miRNAs were obtained. Sixty-five of these were expressed during the transition from precompetent to competent larvae. Thirty-three of these were upregulated and the others were downregulated. Another 123 miRNAs were expressed during the transition from competent to postlarvae. Ninety-six of these were upregulated and the remaining 27 were downregulated. The expression ofmiR-276-y, miR-100-x, miR-183-x, and miR-263-x showed a >100-fold change during development, while the miR-242-x and novel-m0052-3p expression levels changed over 3000-fold. Putative target gene coexpression, gene ontology, and pathway analyses suggest that these miRNAs play important parts in cell proliferation, migration, apoptosis, metabolic regulation, and energy absorption. Twenty miRNAs and their target genes involved in ingestion, digestion, cytoskeleton, cell adhesion, and apoptosis were identified. Nine of them were analyzed with real-time polymerase chain reaction (PCR), which showed an inverse correlation between the miRNAs and their relative expression levels. Our data elucidate the role of miRNAs in R. venosa metamorphic transition and serve as a solid basis for further investigations into regulatory mechanisms of gastropod metamorphosis.
KeywordmiRNA metamorphic transition gastropod larval
DOI10.1534/g3.117.300210
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China (NSFC)[31572636] ; earmarked fund for Modern Agro-industry Technology Research System[CARS-49] ; Primary Research & Development Plan of Shandong Province[2016ZDJS06A02] ; Industry Leading Talent Project of Taishan scholars ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020703]
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000417073900016
PublisherGENETICS SOCIETY AMERICA
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/157374
Collection海洋生态与环境科学重点实验室
Corresponding AuthorZhang, Tao; Wang, Hai-yan
Affiliation1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Ocean Univ China, Coll Fisheries, Qingdao 266001, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Song, Hao,Qi, Lu,Zhang, Tao,et al. Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)[J]. G3-GENES GENOMES GENETICS,2017,7(12):3999-4008.
APA Song, Hao,Qi, Lu,Zhang, Tao,&Wang, Hai-yan.(2017).Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa).G3-GENES GENOMES GENETICS,7(12),3999-4008.
MLA Song, Hao,et al."Understanding microRNA Regulation Involved in the Metamorphosis of the Veined Rapa Whelk (Rapana venosa)".G3-GENES GENOMES GENETICS 7.12(2017):3999-4008.
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