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High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers
Yi, Yunhai1,2; You, Xinxin2; Bian, Chao2; Chen, Shixi3,4; Lv, Zhao5; Qiu, Limei5; Shi, Qiong1,2,6
Source PublicationMARINE DRUGS
Corresponding AuthorShi, Qiong(
AbstractWidespread existence of antimicrobial peptides (AMPs) has been reported in various animals with comprehensive biological activities, which is consistent with the important roles of AMPs as the first line of host defense system. However, no big-data-based analysis on AMPs from any fish species is available. In this study, we identified 507 AMP transcripts on the basis of our previously reported genomes and transcriptomes of two representative amphibious mudskippers, Boleophthalmus pectinirostris (BP) and Periophthalmus magnuspinnatus (PM). The former is predominantly aquatic with less time out of water, while the latter is primarily terrestrial with extended periods of time on land. Within these identified AMPs, 449 sequences are novel; 15 were reported in BP previously; 48 are identically overlapped between BP and PM; 94 were validated by mass spectrometry. Moreover, most AMPs presented differential tissue transcription patterns in the two mudskippers. Interestingly, we discovered two AMPs, hemoglobin 1 and amylin, with high inhibitions on Micrococcus luteus. In conclusion, our high-throughput screening strategy based on genomic and transcriptomic data opens an efficient pathway to discover new antimicrobial peptides for ongoing development of marine drugs.
Keywordantimicrobial peptide (AMP) high-throughput identification amphibious mudskipper antimicrobial activity
Indexed BySCI
Funding ProjectShenzhen Special Program for Development of Emerging Strategic Industries[JSGG20170412153411369] ; Zhenjiang Leading Talent Program for Innovation and Entrepreneurship
WOS Research AreaPharmacology & Pharmacy
WOS SubjectChemistry, Medicinal
WOS IDWOS:000416600200034
PublisherMDPI AG
Citation statistics
Cited Times:10[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorShi, Qiong
Affiliation1.Univ Chinese Acad Sci, IBGI Educ Ctr, Shenzhen 518083, Peoples R China
2.BGI, Shenzhen Key Lab Marine Gen, Guangdong Prov Key Lab Mol Breeding Marine Econ A, BGI Acad Marine Sci,BGI Marine, Shenzhen 518083, Peoples R China
3.Xiamen Univ, Coll Ocean & Earth Sci, State Key Lab Marine Environm Sci, Xiamen 361102, Peoples R China
4.Xiamen Univ, Fujian Collaborat Innovat Ctr Exploitat & Utiliza, Xiamen 361102, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
6.Shenzhen Univ, Coll Life Sci & Oceanog, Lab Aquat Genom, Shenzhen 518060, Peoples R China
Recommended Citation
GB/T 7714
Yi, Yunhai,You, Xinxin,Bian, Chao,et al. High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers[J]. MARINE DRUGS,2017,15(11):18.
APA Yi, Yunhai.,You, Xinxin.,Bian, Chao.,Chen, Shixi.,Lv, Zhao.,...&Shi, Qiong.(2017).High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers.MARINE DRUGS,15(11),18.
MLA Yi, Yunhai,et al."High-Throughput Identification of Antimicrobial Peptides from Amphibious Mudskippers".MARINE DRUGS 15.11(2017):18.
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