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Combined Proteome and Toxicology Approach Reveals the Lethality of Venom Toxins from Jellyfish Cyanea nozakii
Li, Rongfeng1,2,3; Yu, Huahua1,2,3; Yue, Yang1,2,3; Li, Pengcheng1,2,3
2018-11-01
Source PublicationJOURNAL OF PROTEOME RESEARCH
ISSN1535-3893
Volume17Issue:11Pages:3904-3913
Corresponding AuthorLi, Rongfeng(rongfengli@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
AbstractJellyfish are a type of poisonous cnidarian invertebrate that secrete lethal venom for predation or defense. Human beings often become victims of jellyfish stings accidentally while swimming or fishing and suffer severe pain, itching, swelling, inflammation, shock, and even death. Jellyfish venom is composed of various toxins, and the lethal toxin is the most toxic and hazardous component of the venom, which is responsible for deaths caused by jellyfish stings and envenomation. Our previous study revealed many toxins in jellyfish venom, including phospholipase A2, metalloproteinase, and protease inhibitors. However, it is still unknown which type of toxin is lethal and how it works. Herein a combined toxicology analysis, proteome strategy, and purification approach was employed to investigate the lethality of the venom of the jellyfish Cyanea nozakii. Toxicity analysis revealed that cardiotoxicity including acute myocardial infarction and a significant decrease in both heart rate and blood pressure is the primary cause of death. Purified lethal toxin containing a fraction of jellyfish venom was subsequently subjected to proteome analysis and bioinformation analysis. A total of 316 and 374 homologous proteins were identified, including phospholipase A2-like toxins and metalloprotease-like toxins. Furthermore, we confirmed that the lethality of the jellyfish venom is related to metalloproteinase activity but without any phospholipase A2 activity or hemolytic activity. Altogether, this study not only provides a comprehensive understanding of the lethal mechanism of jellyfish venom but also provides very useful information for the therapeutic or rescue strategy for severe jellyfish stings.
Keywordproteome jellyfish Cyanea nozakii venom CnV CnLF CnTX-1 CnTX-2
DOI10.1021/acs.jproteome.8b00568
Indexed BySCI
Language英语
Funding ProjectNational Natural Science Foundation of China[41876164] ; National Natural Science Foundation of China[41776163] ; National Key RD Plan[2017YFE0111100-04] ; Key Research and Development Plan of Shandong Province[2017GHY215003] ; Qingdao Science and Technology Project[17-3-3-21 nsh]
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemical Research Methods
WOS IDWOS:000449443000026
PublisherAMER CHEMICAL SOC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qdio.ac.cn/handle/337002/156346
Collection实验海洋生物学重点实验室
Corresponding AuthorLi, Rongfeng; Li, Pengcheng
Affiliation1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Biol Prod, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
First Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Corresponding Author AffilicationInstitute of Oceanology, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Li, Rongfeng,Yu, Huahua,Yue, Yang,et al. Combined Proteome and Toxicology Approach Reveals the Lethality of Venom Toxins from Jellyfish Cyanea nozakii[J]. JOURNAL OF PROTEOME RESEARCH,2018,17(11):3904-3913.
APA Li, Rongfeng,Yu, Huahua,Yue, Yang,&Li, Pengcheng.(2018).Combined Proteome and Toxicology Approach Reveals the Lethality of Venom Toxins from Jellyfish Cyanea nozakii.JOURNAL OF PROTEOME RESEARCH,17(11),3904-3913.
MLA Li, Rongfeng,et al."Combined Proteome and Toxicology Approach Reveals the Lethality of Venom Toxins from Jellyfish Cyanea nozakii".JOURNAL OF PROTEOME RESEARCH 17.11(2018):3904-3913.
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